Cdk3, a conjugation-specific cyclin-dependent kinase, is essential for the initiation of meiosis in Tetrahymena thermophila
文献类型：期刊论文
| 作者 | Yan, Guan-xiong1,2; Zhang, Jing1,2; Shodhan, Anura3; Tian, Miao1,3,4; Miao, Wei1 |
| 刊名 | CELL CYCLE
 |
| 出版日期 | 2016 |
| 卷号 | 15期号:18页码:2506-2514 |
| 关键词 | conjugation cyclin-dependent kinase meiosis initiation sexual reproduction Tetrahymena thermophila |
| ISSN号 | 1538-4101 |
| 英文摘要 | Meiosis is an important process in sexual reproduction. Meiosis initiation has been found to be highly diverse among species. In yeast, it has been established that cyclin-dependent kinases (Cdks) and cyclins are essential components in the meiosis initiation pathway. In this study, we identified 4 Cdks in the model ciliate, Tetrahymena thermophila, and we found one of them, Cdk3, which is specifically expressed during early conjugation, to be essential for meiosis initiation. Cdk3 deletion led to arrest at the pair formation stage of conjugation. We then confirmed that Cdk3 acts upstream of double-strand break (DSB) formation. Moreover, we detected that Cdk3 is necessary for the expression of many genes involved in early meiotic events. Through proteomic quantification of phosphorylation, co-expression analysis and RNA-Seq analyses, we identified a conjugation-specific cyclin, Cyc2, which most likely partners with Cdk3 to initiate meiosis. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Cell Biology |
| 研究领域[WOS] | Cell Biology |
| 关键词[WOS] | CELL-CYCLE ; DNA-REPLICATION ; PROTEIN-KINASE ; GENE ONTOLOGY ; FISSION YEAST ; RNA-SEQ ; PHOSPHORYLATION ; IME2 ; ACTIVATION ; GERMLINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000384022200021 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/28357]  |
| 专题 | 水生生物研究所_水生生物多样性与资源保护研究中心_学位论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ Vienna, Dept Chromosome Biol, Ctr Mol Biol, Vienna, Austria 4.Univ Vienna, Max F Perutz Labs, Ctr Mol Biol, Vienna, Austria |
| 推荐引用方式 GB/T 7714 | Yan, Guan-xiong,Zhang, Jing,Shodhan, Anura,et al. Cdk3, a conjugation-specific cyclin-dependent kinase, is essential for the initiation of meiosis in Tetrahymena thermophila[J]. CELL CYCLE,2016,15(18):2506-2514. |
| APA | Yan, Guan-xiong,Zhang, Jing,Shodhan, Anura,Tian, Miao,&Miao, Wei.(2016).Cdk3, a conjugation-specific cyclin-dependent kinase, is essential for the initiation of meiosis in Tetrahymena thermophila.CELL CYCLE,15(18),2506-2514. |
| MLA | Yan, Guan-xiong,et al."Cdk3, a conjugation-specific cyclin-dependent kinase, is essential for the initiation of meiosis in Tetrahymena thermophila".CELL CYCLE 15.18(2016):2506-2514. |
入库方式： OAI收割
来源：水生生物研究所
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