Methionine hydroxy analogue enhanced fish immunity via modulation of NF-kappa B, TOR, MLCK, MAPKs and Nrf2 signaling in young grass carp (Ctenopharyngodon idella)
文献类型：期刊论文
| 作者 | Pan, Fei-Yu1; Feng, Lin1,2,3; Jiang, Wei-Dan1,2,3; Jiang, Jun1,2,3; Wu, Pei1,2,3; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5; Zhou, Xiao-Qiu1,2,3 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2016-09-01 |
| 卷号 | 56页码:208-228 |
| 关键词 | Methionine hydroxy analogue Ctenopharyngodon idella Immune function Structural integrity Immune organs Efficacy |
| ISSN号 | 1050-4648 |
| 英文摘要 | Our study investigated the effect of dietary methionine hydroxy analogue (MHA) on growth and immunity (head kidney, spleen and skin) of young grass carp (Ctenopharyngodon idella). A total of 630 grass carp (259.70 +/- 0.47 g) were fed graded levels of MHA (0, 2.4, 4.4, 6.4, 8.5 and 10.5 g/kg diet) and one DL-methionine (DLM) group (6.4 g/kg diet) for 8 weeks. At the end of the feeding trial, fish were challenged with Aeromonas hydrophila for 14 days. The results indicated that optimal MHA increased lysozyme (LZ) and acid phosphatase (ACP) activities, complement 3 (C3), C4 and immunoglobulin M (IgM) contents and up-regulated mRNA levels of liver expressed antimicrobial peptide 2, hepcidin (head kidney), beta-defensin1 in the immune organs (P < 0.05), suggesting that MHA could enhance antimicrobial ability of fish. Meanwhile, optimal MHA enhanced the immune function of immune organs via down-regulating pro inflammatory cytokines mRNA levels and up-regulated anti-inflammatory cytokines mRNA levels, which might be attributed to the down-regulation of nuclear factor kappa B p65, c-Rel, I kappa B kinase beta, p38 mitogen activated protein kinase, eIF4E-binding protein1 (4E-BP1) and 4E-BP2 mRNA levels and up-regulation of inhibitor of kappa B alpha, ribosomal protein S6 kinase 1 and target of rapamycin mRNA levels (P < 0.05). In addition, optimal MHA improved cellular structure integrity of immune organs via repressing death receptor and mitochondria pathways induced apoptosis, which might be related to the down-regulation of c-Jun-N-terminal kinase mRNA levels (P < 0.05). Simultaneously, optimal MHA improved cellular structure integrity of immune organs via elevating glutathione contents, antioxidant enzymes activities and corresponding isoforms mRNA levels to attenuate oxidative damage, which might be to the up regulation of NF-E2-related factor 2 mRNA levels and down-regulation of Kelch-like ECH-associating protein 1a mRNA levels (P < 0.05). Besides, optimal MHA improved intercellular structure integrity of immune organs via up-regulating the mRNA levels of intercellular tight junctions-related genes, which might be owing to the down-regulation of myosin light chain kinase mRNA levels (P < 0.05). In conclusion, MHA exerted a positive effect on the immune function and structural integrity of immune organs in fish. Furthermore, according to the positive effect, MHA was superior to DLM in grass carp. However, based on the growth performance, the efficacy of MHA relative to DLM was 97%. Finally, on the premise of the basal diet containing 4.01 g/kg methionine, the optimal MHA supplementation levels based on feed intake, PWG, defense against skin hemorrhage and lesion, 12 and ACP activities, IgM content, against malondialdehyde, protein carbonyl and ROS in the head kidney of young grass carp were 5.07, 5.21, 5.76, 5.90, 5.88, 5.80, 6.22, 5.68 and 6.85 g/kg diet, respectively. (C) 2016 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | 2-HYDROXY-4-(METHYLTHIO) BUTANOIC ACID ; INTESTINAL ENZYMES ACTIVITIES ; NITRIC-OXIDE SYNTHASE ; DIETARY BETA-GLUCAN ; CYPRINUS-CARPIO ; GENE-EXPRESSION ; JUVENILE JIAN ; VAR. JIAN ; GROWTH-PERFORMANCE ; DISEASE RESISTANCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000383292200021 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/28366]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_学位论文 |
| 作者单位 | 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Peoples R China 2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ, Key Lab Sichuan Prov, Chengdu 611130, Peoples R China 3.Sichuan Agr Univ, Key Lab Anim Dis Resistance Nutr China, Minist Educ, Chengdu 611130, Peoples R China 4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pan, Fei-Yu,Feng, Lin,Jiang, Wei-Dan,et al. Methionine hydroxy analogue enhanced fish immunity via modulation of NF-kappa B, TOR, MLCK, MAPKs and Nrf2 signaling in young grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2016,56:208-228. |
| APA | Pan, Fei-Yu.,Feng, Lin.,Jiang, Wei-Dan.,Jiang, Jun.,Wu, Pei.,...&Liu, Yang.(2016).Methionine hydroxy analogue enhanced fish immunity via modulation of NF-kappa B, TOR, MLCK, MAPKs and Nrf2 signaling in young grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,56,208-228. |
| MLA | Pan, Fei-Yu,et al."Methionine hydroxy analogue enhanced fish immunity via modulation of NF-kappa B, TOR, MLCK, MAPKs and Nrf2 signaling in young grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 56(2016):208-228. |
入库方式： OAI收割
来源：水生生物研究所
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