The kinetics and protection of the antiviral state induced by recombinant iIFN1a in rainbow trout against infectious hematopoietic necrosis virus
文献类型：期刊论文
| 作者 | Cao, Yongsheng1,2; Xu, Liming1; LaPatra, Scott E.3; Zhao, Jingzhuang1; Liu, Miao1; Liu, Hongbai1; Lu, Tongyan1; Zhang, Qiya2 |
| 刊名 | MOLECULAR IMMUNOLOGY
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 76页码:55-61 |
| 关键词 | ilFN1a of rainbow trout Prokaryotic expressing Antiviral state against IHNV in vivo Variant protection |
| ISSN号 | 0161-5890 |
| 英文摘要 | The iIFN1a (intracellular IFN-a1), that is one of the IFN-a1 variants, was shown to be functional intracellularly and act as a novel defense against an infectious.hematopoietic necrosis virus (IHNV). To determine its antiviral properties, a recombinant iIFN1a was generated in Escherichia coli. Its antiviral activity against IHNV was 1.69 x 10(7) U/mg in CHSE-214 cells. Additionally, iIFN1a was capable of inducing comparable levels of IRF-1, IRF-2, IFN-1, IFN-gamma and Mx transcription in head kidney, spleen and liver tissues at an early time point (6 h), that was followed by a rapid decline 24 h after induction. The recombinant protein also elicited protection against IHNV in vivo. At 6 and 24 h after induction there was 100% protection against the virus, however, at 48 and 72 h the protection decreased to 57 and 40%, respectively. The in vivo protection kinetics correlated with the kinetics of gene expression. The results of this study provide details of the antiviral state that was induced by iIFN1a in vivo for the first time. Additionally, this information will facilitate the development of this recombinant protein as a potential anti-viral treatment and/or adjuvant. (C) 2016 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Immunology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Immunology |
| 关键词[WOS] | ONCORHYNCHUS-MYKISS ; INTERFERON-ALPHA ; ESCHERICHIA-COLI ; DNA VACCINATION ; IN-VITRO ; FISH ; IFN ; EXPRESSION ; PATHWAY ; MECHANISMS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000381836900008 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/28378]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_学位论文 |
| 作者单位 | 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Dept Aquaculture, Harbin 150070, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 3.Clear Springs Foods Inc, Div Res, POB 712, Buhl, ID 83316 USA |
| 推荐引用方式 GB/T 7714 | Cao, Yongsheng,Xu, Liming,LaPatra, Scott E.,et al. The kinetics and protection of the antiviral state induced by recombinant iIFN1a in rainbow trout against infectious hematopoietic necrosis virus[J]. MOLECULAR IMMUNOLOGY,2016,76:55-61. |
| APA | Cao, Yongsheng.,Xu, Liming.,LaPatra, Scott E..,Zhao, Jingzhuang.,Liu, Miao.,...&Zhang, Qiya.(2016).The kinetics and protection of the antiviral state induced by recombinant iIFN1a in rainbow trout against infectious hematopoietic necrosis virus.MOLECULAR IMMUNOLOGY,76,55-61. |
| MLA | Cao, Yongsheng,et al."The kinetics and protection of the antiviral state induced by recombinant iIFN1a in rainbow trout against infectious hematopoietic necrosis virus".MOLECULAR IMMUNOLOGY 76(2016):55-61. |
入库方式： OAI收割
来源：水生生物研究所
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