EseE of Edwardsiella tarda Augments Secretion of Translocon Protein EseC and Expression of the escC-eseE Operon
文献类型：期刊论文
| 作者 | Yi, Jia1; Xiao, Shui Bing2; Zeng, Zhi Xiong2; Lu, Jin Fang1; Liu, Lu Yi1; Laghari, Zubair Ahmed1; Nie, Pin1; Yu, Hong Bing3; Xie, Hai Xia1 |
| 刊名 | INFECTION AND IMMUNITY
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 84期号:8页码:2336-2344 |
| ISSN号 | 0019-9567 |
| 英文摘要 | Edwardsiella tarda is an important Gram-negative pathogen that employs a type III secretion system (T3SS) to deliver effectors into host cells to facilitate bacterial survival and replication. These effectors are translocated into host cells through a translocon complex composed of three secreted proteins, namely, EseB, EseC, and EseD. The secretion of EseB and EseD requires a chaperone protein called EscC, whereas the secretion of EseC requires the chaperone EscA. In this study, we identified a novel protein (EseE) that also regulates the secretion of EseC. An eseE deletion mutant secreted much less EseC into supernatants, accompanied by increased EseC levels within bacterial cells. We also demonstrated that EseE interacted directly with EseC in a pulldown assay. Interestingly, EseC, EseE, and EscA were able to form a ternary complex, as revealed by pulldown and gel filtration assays. Of particular importance, the deletion of eseE resulted in decreased levels of EseB and EseD proteins in both the bacterial pellet and supernatant fraction. Furthermore, real-time PCR assays showed that EseE positively regulated the transcription of the translocon operon escC-eseE, comprising escC, eseB, escA, eseC, eseD, and eseE. These effects of EseE on the translocon components/operon appeared to have a functional consequence, since the Delta eseE strain was outcompeted by wild-type E. tarda in a mixed infection in blue gourami fish. Collectively, our results demonstrate that EseE not only functions as a chaperone for EseC but also acts as a positive regulator controlling the expression of the translocon operon escC-eseE, thus contributing to the pathogenesis of E. tarda in fish. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Immunology ; Infectious Diseases |
| 研究领域[WOS] | Immunology ; Infectious Diseases |
| 关键词[WOS] | III SECRETION ; SALMONELLA-TYPHIMURIUM ; VIRULENCE GENES ; YERSINIA-ENTEROCOLITICA ; 2-COMPONENT SYSTEM ; SHIGELLA-FLEXNERI ; CHAPERONE ; FISH ; EFFECTORS ; INVF |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000380746400017 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/28379]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_学位论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei Province, Peoples R China 2.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan, Hubei Province, Peoples R China 3.Child & Family Res Inst, Vancouver, BC, Canada |
| 推荐引用方式 GB/T 7714 | Yi, Jia,Xiao, Shui Bing,Zeng, Zhi Xiong,et al. EseE of Edwardsiella tarda Augments Secretion of Translocon Protein EseC and Expression of the escC-eseE Operon[J]. INFECTION AND IMMUNITY,2016,84(8):2336-2344. |
| APA | Yi, Jia.,Xiao, Shui Bing.,Zeng, Zhi Xiong.,Lu, Jin Fang.,Liu, Lu Yi.,...&Xie, Hai Xia.(2016).EseE of Edwardsiella tarda Augments Secretion of Translocon Protein EseC and Expression of the escC-eseE Operon.INFECTION AND IMMUNITY,84(8),2336-2344. |
| MLA | Yi, Jia,et al."EseE of Edwardsiella tarda Augments Secretion of Translocon Protein EseC and Expression of the escC-eseE Operon".INFECTION AND IMMUNITY 84.8(2016):2336-2344. |
入库方式： OAI收割
来源：水生生物研究所
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