Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny.
文献类型:期刊论文
作者 | Li, Xiaochuang1,2; Li, Shouchun3; Kong, Renqiu1; Li, Renhui1 |
刊名 | HARMFUL ALGAE
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出版日期 | 2016-07-01 |
卷号 | 57页码:88-97 |
关键词 | Cylindrospermopsis ITS-L Phylogeny Raphidiopsis Taxonomy |
ISSN号 | 1568-9883 |
英文摘要 | The cyanobacterial genera Raphidiopsis and Cylindrospermopsis morphologically resembled each other and are difficult to distinguish, especially when heterocytes do not form in Cylindrospermopsis species. The phylogeny based on multiple gene loci sequences in previous studies cannot divide the strains of these two genera into separate clusters. In the present study, four gene loci 16S rRNA, ITS-L, cpcBA-IGS and rpoC1, sequenced from many Chinese strains (44 Cylindrospermopsis strains and 16 Raphidiopsis strains), were analyzed to infer the phylogenetic relationship between the two genera. According to the 16S rRNA, cpcBA-IGS and rpoC1 gene phylogeny, Cylindrospermopsis and Raphidiopsis strains are intermixed and divide into two or three groups. By contrast, the ITS-L analysis implied that they respectively form two separate and unique clusters. A fragment of 7 bp (RAGAAACT) in ITS-L was conserved for Raphidiopsis strains, which could be distinguished from Cylindrospermopsis strains. A pair of primers targeting the ITS-L fragment specific to Raphidiopsis strains were designed and further verified. (C) 2016 Elsevier B.V. All rights reserved. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Marine & Freshwater Biology |
研究领域[WOS] | Marine & Freshwater Biology |
关键词[WOS] | POPULATION-DYNAMICS ; SUBBA RAJU ; MEDITERRANEA SKUJA ; FLORIDA LAKES ; 1ST REPORT ; RACIBORSKII ; RESERVOIRS ; DOMINANCE ; SEQUENCES ; RELEASE |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000381544600009 |
源URL | [http://ir.ihb.ac.cn/handle/342005/28345] ![]() |
专题 | 水生生物研究所_藻类生物学及应用研究中心_学位论文 |
作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Jiangxi Normal Univ, Coll Life Sci, Nanchang 330027, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Xiaochuang,Li, Shouchun,Kong, Renqiu,et al. Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny.[J]. HARMFUL ALGAE,2016,57:88-97. |
APA | Li, Xiaochuang,Li, Shouchun,Kong, Renqiu,&Li, Renhui.(2016).Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny..HARMFUL ALGAE,57,88-97. |
MLA | Li, Xiaochuang,et al."Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny.".HARMFUL ALGAE 57(2016):88-97. |
入库方式: OAI收割
来源:水生生物研究所
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