Changes in endogenous hormones and H2O2 burst during shoot organogenesis in TDZ-treated Saussurea involucrate explants
文献类型：期刊论文
| 作者 | Guo, Bin1; He, Wei1; Zhao, Yan2,3; Wu, Yandi1; Fu, Yanping1; Guo, Juan4 ; Wei, Yahui1
|
| 刊名 | plant cell tissue and organ culture
 |
| 出版日期 | 2017 |
| 卷号 | 128期号:1页码:1-8 |
| 关键词 | Saussurea involucrata Thidiazuron Shoot organogenesis Endogenous plant growth regulators H2O2 |
| ISSN号 | 0167-6857 |
| 产权排序 | 4 |
| 英文摘要 | thidiazuron [n-phenyl-n-(1, 2, 3-thidiazol-5-yl) urea, tdz] treatment significantly improved shoot morphogenesis of saussurea involucrata kar. et kir (s. involucrata) leaf explants. the biochemical mechanisms underlying tdz-induced shoot organogenesis were investigated by measuring endogenous plant growth hormones, h2o2, as well as the activities of superoxide dismutase (sod) and catalase (cat). the levels of endogenous gibberellic a3 (ga(3)) and zeatin (zt) significantly increased in leaf explants subject to a 28-day treatment than the controls. however, extending exposure time to tdz inhibited ga(3) accumulation. at the same time, the sod activity increased significantly until the 28th of tdz treatment time and the cat activity reduced simultaneously, which was closely linked with the significant increase in h2o2 concentrations in the explants. and there was a sharply promotion after the 35 day of culture time if the plant tissue was always in medium contained, which was in company with the cell activity decreased. we propose that a combination of increased ga(3), zt, and h2o2 concentration is the basis for the enhanced shoot morphogenesis in response to tdz treatment. these results provide a starting point for an improved understanding of the biochemical mechanisms underlying tdz-induced shoot organogenesis of s. involucrata. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 细胞生物学 |
| 类目[WOS] | biotechnology & applied microbiology ; plant sciences |
| 研究领域[WOS] | biotechnology & applied microbiology ; plant sciences |
| 关键词[WOS] | induced somatic embryogenesis ; antioxidant enzymes ; plant-regeneration ; hydrogen-peroxide ; callus-cultures ; thidiazuron ; growth ; cytokinins ; regulator ; embryos |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000391942400001 |
| 源URL | [http://ir.opt.ac.cn/handle/181661/28415]  |
| 专题 | 西安光学精密机械研究所_网络信息中心 |
| 作者单位 | 1.Northwest Univ Xian, Minist Educ, Key Lab Resource Biol & Biotechnol Western China, Shaanxi Prov Key Lab Biotechnol,Coll Life Sci, Xian 710069, Shaanxi, Peoples R China 2.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Key Lab Agroprod Qual & Safety, Beijing, Peoples R China 3.Minist Agr, Key Lab Agroprod Qual & Safety, Beijing, Peoples R China 4.Chinese Acad Sci, Xian Inst Opt & Precis Mech, Xian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Bin,He, Wei,Zhao, Yan,et al. Changes in endogenous hormones and H2O2 burst during shoot organogenesis in TDZ-treated Saussurea involucrate explants[J]. plant cell tissue and organ culture,2017,128(1):1-8. |
| APA | Guo, Bin.,He, Wei.,Zhao, Yan.,Wu, Yandi.,Fu, Yanping.,...&Wei, Yahui.(2017).Changes in endogenous hormones and H2O2 burst during shoot organogenesis in TDZ-treated Saussurea involucrate explants.plant cell tissue and organ culture,128(1),1-8. |
| MLA | Guo, Bin,et al."Changes in endogenous hormones and H2O2 burst during shoot organogenesis in TDZ-treated Saussurea involucrate explants".plant cell tissue and organ culture 128.1(2017):1-8. |
入库方式： OAI收割
来源：西安光学精密机械研究所
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