Inhibition of biofilm in Bacillus amyloliquefaciens Q-426 by diketopiperazines
文献类型：期刊论文
| 作者 | Wang, Jian-Hua1,3; Yang, Cui-Yun2; Fang, Sheng-Tao2; Lu, Jian1; Quan, Chun-Shan4 |
| 刊名 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2016-09-01 |
| 卷号 | 32期号:9 |
| 关键词 | Biofilm Diketopiperazines (DKPs) Extracellular polymeric substance (EPS) Real-time PCR Bacillus amyloliquefaciens Q-426 |
| 英文摘要 | Biofilm formation can make significant effects on bacteria habits and biological functions. In this study, diketopiperazines (DKPs) produced by strain of Bacillus amyloliquefaciens Q-426 was found to inhibit biofilm formed in the gas-liquid interface. Four kinds of DKPs were extracted from B. amyloliquefaciens Q-426, and we found that 0.04 mg ml(-1) DKPs could obviously inhibit the biofilm formation of the strain. DKPs produced by B. amyloliquefaciens Q-426 made a reduction on extracellular polymeric substance (EPS) components, polysaccharides, proteins, DNAs, etc. Real-time PCR was performed to determine that whether DKPs could make an obvious effect on the expression level for genes related to biofilm formation in the strain. The relative expression level of genes tasA, epsH, epsG and remB which related to proteins, extracellular matrix, and polysaccharides, were downregulated with 0.04 mg ml(-1) DKPs, while the expression level of nuclease gene nuc was significantly upregulated. The quantitative results of the mRNA expression level for these genes concerted with the quantitative results on EPS levels. All of the experimental results ultimately indicated that DKPs could inhibit the biofilm formation of the strain B. amyloliquefaciens Q-426. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000382148200004 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/135828]  |
| 专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao, Peoples R China 4.Dalian Nationalities Univ, Life Sci Coll, Dalian 116600, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jian-Hua,Yang, Cui-Yun,Fang, Sheng-Tao,et al. Inhibition of biofilm in Bacillus amyloliquefaciens Q-426 by diketopiperazines[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2016,32(9). |
| APA | Wang, Jian-Hua,Yang, Cui-Yun,Fang, Sheng-Tao,Lu, Jian,&Quan, Chun-Shan.(2016).Inhibition of biofilm in Bacillus amyloliquefaciens Q-426 by diketopiperazines.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,32(9). |
| MLA | Wang, Jian-Hua,et al."Inhibition of biofilm in Bacillus amyloliquefaciens Q-426 by diketopiperazines".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 32.9(2016). |
入库方式： OAI收割
来源：海洋研究所
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