Understanding mechanism of sea cucumber Apostichopus japonicus aestivation: Insights from TMT-based proteomic study
文献类型：期刊论文
| 作者 | Chen, Muyan1; Li, Xingke1; Zhu, Aijun1; Storey, Kenneth B.2; Sun, Lina3; Gao, Tianxiang4; Wang, Tianming5 |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
 |
| 出版日期 | 2016-09-01 |
| 卷号 | 19页码:78-89 |
| 关键词 | Aestivation Sea cucumber Proteome Tandem mass tag Post-transcriptional regulation |
| 英文摘要 | Marine invertebrate aestivation is a unique strategy for summer survival in response to hot marine conditions. The sea cucumber, Apostichopus japonicus, is an excellent model marine invertebrate for studies of environmentally-induced aestivation. In the present study, we used a tandem mass tag (TMT)-coupled LC-MS/MS approach to identify and quantify the global proteome expression profile over the aestivation-arousal cycle of A. japonicus. A total of 3920 proteins were identified from the intestine of sea cucumber. Among them, 630 proteins showed significant differential expression when comparing three conditions of sea cucumbers: non aestivating (active), deep-aestivation (at least 15 days of continuous aestivation), and arousal after aestivation (renewed moving and feeding). Sea cucumbers in deep aestivation showed substantial differentially expressed proteins (143 up-regulated and 267 down-regulated proteins compared with non-aestivating controls). These differentially expressed proteins suggested that protein and phospholipid probably are major fuel sources during hypometabolism and a general attenuation of carbohydrate metabolism was observed during deep aestivation. Differentially expressed proteins also provided the first global picture of a shift in protein synthesis, protein folding, DNA binding, apoptosis, cellular transport and signaling, and cytoskeletal proteins during deep aestivation in sea cucumbers. A comparison of arousal from aestivation with deep aestivation, revealed a general reversal of the changes that occurred in aestivation for most proteins. Western blot detection further validated the significant up-regulation of HSP70 and down-regulation of methyltransferase-like protein 7A-like in deep-aestivation. Our results suggest that there is substantial post-transcriptional regulation of proteins during the aestivation arousal cycle in sea cucumbers. (C) 2016 Elsevier Inc. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000382416300009 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/135755]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Ocean Univ China, Coll Fisheries, Qingdao, Peoples R China 2.Carleton Univ, Inst Biochem, 1125 Colonel Dr, Ottawa, ON K1S 5B6, Canada 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 4.Zhejiang Ocean Univ, Fishery Coll, Zhoushan, Peoples R China 5.Zhejiang Ocean Univ, Marine Sci & Technol Coll, Zhoushan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Muyan,Li, Xingke,Zhu, Aijun,et al. Understanding mechanism of sea cucumber Apostichopus japonicus aestivation: Insights from TMT-based proteomic study[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2016,19:78-89. |
| APA | Chen, Muyan.,Li, Xingke.,Zhu, Aijun.,Storey, Kenneth B..,Sun, Lina.,...&Wang, Tianming.(2016).Understanding mechanism of sea cucumber Apostichopus japonicus aestivation: Insights from TMT-based proteomic study.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,19,78-89. |
| MLA | Chen, Muyan,et al."Understanding mechanism of sea cucumber Apostichopus japonicus aestivation: Insights from TMT-based proteomic study".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 19(2016):78-89. |
入库方式： OAI收割
来源：海洋研究所
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