CsPTX1, a pentraxin of Cynoglossus semilaevis, is an innate immunity factor with antibacterial effects
文献类型：期刊论文
| 作者 | Wang, Ting1,2,3 ; Zhang, Jian1,2
|
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2016-09-01 |
| 卷号 | 56页码:12-20 |
| 关键词 | Pentraxin Cynoglossus semilaevis Innate immunity Bacterial agglutination Antibacterial effect |
| 英文摘要 | Pentraxin 1 (PTX1) is a member of the pentraxin protein family, which plays important roles in the innate immunity of vertebrates. In fish, the biological function of PTX1 is essentially unknown. In this study, we examined the expression and function of a PTX homologue (CsPTX1) from the tongue sole, Cynoglossus semilaevis. CsPTX1 contains 223 amino acids and shares 49.3%-38.8% overall sequence identity with other known fish pentraxins. CsPTX1 is expressed in multiple tissues and is upregulated by bacterial and viral infection. CsPTX1 contains a pentraxin domain, which is known to bind extracellular antigens, and recombinant CsPTX1 (rCsPTX1) bound a wide range of Gram-positive and Gram-negative bacteria. rCsPTX1 also agglutinated all the bacteria tested in a Ca2+-dependent manner and the agglutinating capacity of rCsPTX1 was abolished in the absence of calcium. As well as its ability to agglutinate bacterial cells, rCsPTX1 displayed apparent bacteriostatic activity against Pseudomonas fluorescens in vitro by influencing the permeability of the microbial envelope. When introduced in vivo, rCsPTX1 enhanced the host's resistance to bacterial infection. These results indicate that CsPTX1 is a classic pattern recognition molecule that defends C semilaevis against bacterial infection. (C) 2016 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000383292200002 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/135839]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ting,Zhang, Jian. CsPTX1, a pentraxin of Cynoglossus semilaevis, is an innate immunity factor with antibacterial effects[J]. FISH & SHELLFISH IMMUNOLOGY,2016,56:12-20. |
| APA | Wang, Ting,&Zhang, Jian.(2016).CsPTX1, a pentraxin of Cynoglossus semilaevis, is an innate immunity factor with antibacterial effects.FISH & SHELLFISH IMMUNOLOGY,56,12-20. |
| MLA | Wang, Ting,et al."CsPTX1, a pentraxin of Cynoglossus semilaevis, is an innate immunity factor with antibacterial effects".FISH & SHELLFISH IMMUNOLOGY 56(2016):12-20. |
入库方式： OAI收割
来源：海洋研究所
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