An invertebrate-specific miRNA targeted the ancient cholinergic neuroendocrine system of oyster
文献类型：期刊论文
| 作者 | Chen, Hao1,3 ; Zhou, Zhi1; Wang, Lingling2; Wang, Hao1; Liu, Rui1; Zhang, Huan1; Song, Linsheng1,2
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| 刊名 | OPEN BIOLOGY
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 6期号:8 |
| 关键词 | miRNA miR-2 family choline transporter-like protein acetylcholine cholinergic system immunomodulation |
| 英文摘要 | Acetylcholine (ACh) is the main neurotransmitter in the cholinergic neuroendocrine system and plays an indispensable role in modulating diverse immune responses. As important transporters in choline uptake, choline transporter-like proteins (CTLs) can control ACh synthesis and release indirectly in multiple organisms. In this study, cgi-miR-2d, an invertebrate- specific miRNA in oyster Crassostrea gigas, is proved to repress the synthesis/release of ACh by targeting CgCTL1 and choline uptake in haemocytes during the early stage of pathogen infection. In short, an opposite expression pattern between CgCTL1 and cgi-miR-2d is observed during Vibrio splendidus infection, accompanied by changes in haemolymph ACh. In addition, the expression level of CgCTL1 is found to be significantly repressed after cgi-miR-2d overexpression in vivo, while both haemocyte choline and haemolymph ACh are also decreased simultaneously, similar to the finding in CgCTL1 knock-down assay. As a result, the expression of two tumour necrosis factor-like proteins and the bacteriostatic activity of oyster haemocytes are found to be altered significantly by either gain-offunction cgi-miR-2d or knock-down of CgCTL1. To our knowledge, this is the first miRNA identified in invertebrates that can target the ancient cholinergic system and augment immune response during infection. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000382945100002 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/135841]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Hao,Zhou, Zhi,Wang, Lingling,et al. An invertebrate-specific miRNA targeted the ancient cholinergic neuroendocrine system of oyster[J]. OPEN BIOLOGY,2016,6(8). |
| APA | Chen, Hao.,Zhou, Zhi.,Wang, Lingling.,Wang, Hao.,Liu, Rui.,...&Song, Linsheng.(2016).An invertebrate-specific miRNA targeted the ancient cholinergic neuroendocrine system of oyster.OPEN BIOLOGY,6(8). |
| MLA | Chen, Hao,et al."An invertebrate-specific miRNA targeted the ancient cholinergic neuroendocrine system of oyster".OPEN BIOLOGY 6.8(2016). |
入库方式： OAI收割
来源：海洋研究所
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