Formation and stimuli-directed migration of D. discoideum slugs in microchips
文献类型：期刊论文
| 作者 | Kim, Jinho1; Olsen, Timothy1; Zhuang, Xuye2; Luo, Ji2; Yao, Jun3; Stojanovic, Milan3; Lin, Qiao1 |
| 刊名 | Journal of Medical and Biological Engineering
 |
| 出版日期 | 2013 |
| 卷号 | 33期号:3页码:263-268 |
| ISSN号 | 16090985 |
| 通讯作者 | Lin, Q. (qlin@columbia.edu) |
| 中文摘要 | This paper presents a microfluidic device that geometrically constrains the development of individual Dictyostelium discoideum cells into multicellular organisms (slugs). A microchip for the stimuli-directed migration of slugs is also presented. To demonstrate the formation of slugs in a predetermined shape, a microchip is designed to confine the slugs in the vertical direction. In the microchip, sufficient oxygen is supplied to the cells via a membrane, allowing the formation and adaptation of slugs to the shape of the channel. In addition, the manipulation of slug migration direction in a microchip via external stimuli, such as light and temperature gradients, that induce phototaxis and thermotaxis of slugs, respectively, is demonstrated. To direct slug migration with external stimuli, an optical fiber is used for phototaxis and an electrical wire heater is used for thermotaxis. Experimental results show slug formation in a predefined geometry in the microchip, suggesting that this chip is potentially useful for understanding the relationship between the shape and function of cells or tissue. The controlled migration of slugs demonstrated in the microchips can potentially be employed in biologically based microactuators or microrobots. |
| 英文摘要 | This paper presents a microfluidic device that geometrically constrains the development of individual Dictyostelium discoideum cells into multicellular organisms (slugs). A microchip for the stimuli-directed migration of slugs is also presented. To demonstrate the formation of slugs in a predetermined shape, a microchip is designed to confine the slugs in the vertical direction. In the microchip, sufficient oxygen is supplied to the cells via a membrane, allowing the formation and adaptation of slugs to the shape of the channel. In addition, the manipulation of slug migration direction in a microchip via external stimuli, such as light and temperature gradients, that induce phototaxis and thermotaxis of slugs, respectively, is demonstrated. To direct slug migration with external stimuli, an optical fiber is used for phototaxis and an electrical wire heater is used for thermotaxis. Experimental results show slug formation in a predefined geometry in the microchip, suggesting that this chip is potentially useful for understanding the relationship between the shape and function of cells or tissue. The controlled migration of slugs demonstrated in the microchips can potentially be employed in biologically based microactuators or microrobots. |
| 学科主题 | Fluidic devices - Microactuators - Microorganisms - Optical fibers |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000322734800004 |
| 源URL | [http://ir.ioe.ac.cn/handle/181551/6840]  |
| 专题 | 光电技术研究所_微细加工光学技术国家重点实验室（开放室） |
| 作者单位 | 1.Department of Mechanical Engineering, Columbia University, New York, NY 10027, United States 2.Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 610209, China 3.Division of Clinical Pharmacology and Experimental Therapeutics, Department of Medicine, Columbia University, New York, NY 10032, United States |
| 推荐引用方式 GB/T 7714 | Kim, Jinho,Olsen, Timothy,Zhuang, Xuye,et al. Formation and stimuli-directed migration of D. discoideum slugs in microchips[J]. Journal of Medical and Biological Engineering,2013,33(3):263-268. |
| APA | Kim, Jinho.,Olsen, Timothy.,Zhuang, Xuye.,Luo, Ji.,Yao, Jun.,...&Lin, Qiao.(2013).Formation and stimuli-directed migration of D. discoideum slugs in microchips.Journal of Medical and Biological Engineering,33(3),263-268. |
| MLA | Kim, Jinho,et al."Formation and stimuli-directed migration of D. discoideum slugs in microchips".Journal of Medical and Biological Engineering 33.3(2013):263-268. |
入库方式： OAI收割
来源：光电技术研究所
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