Aberration correction during real time in vivo imaging of bone marrow with sensorless adaptive optics confocal microscope
文献类型：期刊论文
| 作者 | Wang, Zhibin1,2,3; Wei, Dan4; Wei, Ling1,2; He, Yi1,2; Shi, Guohua1,2; Wei, Xunbin4; Zhang, Yudong1,2 |
| 刊名 | Journal of Biomedical Optics
 |
| 出版日期 | 2014 |
| 卷号 | 19期号:8页码:086009 |
| ISSN号 | 10833668 |
| 通讯作者 | Shi, G. (ioe_eye@126.com) |
| 中文摘要 | We have demonstrated adaptive correction of specimen-induced aberration during in vivo imaging of mouse bone marrow vasculature with confocal fluorescence microscopy. Adaptive optics system was completed with wavefront sensorless correction scheme based on stochastic parallel gradient descent algorithm. Using image sharpness as the optimization metric, aberration correction was performed based upon Zernike polynomial modes. The experimental results revealed the improved signal and resolution leading to a substantially enhanced image contrast with aberration correction. The image quality of vessels at 38-and 75-im depth increased three times and two times, respectively. The corrections allowed us to detect clearer bone marrow vasculature structures at greater contrast and improve the signal-to-noise ratio. © 2014 Society of Photo-Optical Instrumentation Engineers. |
| 英文摘要 | We have demonstrated adaptive correction of specimen-induced aberration during in vivo imaging of mouse bone marrow vasculature with confocal fluorescence microscopy. Adaptive optics system was completed with wavefront sensorless correction scheme based on stochastic parallel gradient descent algorithm. Using image sharpness as the optimization metric, aberration correction was performed based upon Zernike polynomial modes. The experimental results revealed the improved signal and resolution leading to a substantially enhanced image contrast with aberration correction. The image quality of vessels at 38-and 75-im depth increased three times and two times, respectively. The corrections allowed us to detect clearer bone marrow vasculature structures at greater contrast and improve the signal-to-noise ratio. © 2014 Society of Photo-Optical Instrumentation Engineers. |
| 学科主题 | Adaptive optics - Fluorescence - Fluorescence microscopy - Gradient methods - Mammals |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000341250300024 |
| 源URL | [http://ir.ioe.ac.cn/handle/181551/6378]  |
| 专题 | 光电技术研究所_自适应光学技术研究室（八室） |
| 作者单位 | 1.Chinese Academy of Sciences, Key Laboratory on Adaptive Optics, Chengdu 610209, China 2.Chinese Academy of Sciences, Institute of Optics and Electronics, Laboratory on Adaptive Optics, Chengdu 610209, China 3.University of Chinese Academy of Sciences, Beijing 100039, China 4.Shanghai Jiao Tong University, School of Biomedical Engineering and Med-X Research Institute, 1954 Huashan Road, Shanghai 200030, China |
| 推荐引用方式 GB/T 7714 | Wang, Zhibin,Wei, Dan,Wei, Ling,et al. Aberration correction during real time in vivo imaging of bone marrow with sensorless adaptive optics confocal microscope[J]. Journal of Biomedical Optics,2014,19(8):086009. |
| APA | Wang, Zhibin.,Wei, Dan.,Wei, Ling.,He, Yi.,Shi, Guohua.,...&Zhang, Yudong.(2014).Aberration correction during real time in vivo imaging of bone marrow with sensorless adaptive optics confocal microscope.Journal of Biomedical Optics,19(8),086009. |
| MLA | Wang, Zhibin,et al."Aberration correction during real time in vivo imaging of bone marrow with sensorless adaptive optics confocal microscope".Journal of Biomedical Optics 19.8(2014):086009. |
入库方式： OAI收割
来源：光电技术研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。