Transcriptome Analysis Identifies Candidate Genes Related to Triacylglycerol and Pigment Biosynthesis and Photoperiodic Flowering in the Ornamental and Oil-Producing Plant, Camellia reticulata (Theaceae)
文献类型：期刊论文
| 作者 | Yao, Qiu-Yang1,2; Huang, Hui1; Tong, Yan1; Xia, En-Hua1,2 ; Gao, Li-Zhi1
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| 刊名 | FRONTIERS IN PLANT SCIENCE
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| 出版日期 | 2016-02-23 |
| 卷号 | 7 |
| 关键词 | Camellia reticulata transcriptome triacylglycerol biosynthesis photoperiodic flowering pathway pigment biosynthesis EST-SSRs |
| 英文摘要 | Camellia reticulate, which is native to Southwest China, is famous for its ornamental flowers and high-quality seed oil. However, the lack of genomic information for this species has largely hampered our understanding of its key pathways related to oil production, photoperiodic flowering process and pigment biosynthesis. Here, we first sequenced and characterized the transcriptome of a diploid C. reticulate in an attempt to identify genes potentially involved in triacylglycerol biosynthesis (TAGBS), photoperiodic flowering, flavonoid biosynthesis (FIaBS), carotenoid biosynthesis (CrtBS) pathways. De novo assembly of the transcriptome provided a catalog of 141,460 unigenes with a total length of similar to 96.1 million nucleotides (Mnt) and an N50 of 1080 nt. Of them, 22,229 unigenes were defined as differentially expressed genes (DEGs) across five sequenced tissues. A large number of annotated genes in C. reticulate were found to have been duplicated, and differential expression patterns of these duplicated genes were commonly observed across tissues, such as the differential expression of SOC1_a, SOC1_b, and SOC1_c in the photoperiodic flowering pathway. Up regulation of SAD_a and FATA genes and down-regulation of FAD2_a gene in the TAGBS pathway in seeds may be relevant to the ratio of monounsaturated fatty acid (MUFAs) to polyunsaturated fatty acid (PUFAs) in seed oil. MYBF1, a transcription regulator gene of the FIaBS pathway, was found with great sequence variation and alteration of expression patterns, probably resulting in functionally evolutionary differentiation in C. reticulate. MYBALa and some anthocyanin-specific biosynthetic genes in the FIaBS pathway were highly expressed in both flower buds and flowers, suggesting important roles of anthocyanin biosynthesis in flower development. Besides, a total of 40,823 expressed sequence tag simple sequence repeats (EST-SSRs) were identified in the C. reticulate transcriptome, providing valuable marker resources for further basic and applied researches on this economically important Camellia plant. |
| 类目[WOS] | Plant Sciences |
| 研究领域[WOS] | Plant Sciences |
| 关键词[WOS] | DEVELOPING GRAPE BERRIES ; FATTY-ACID-COMPOSITION ; ARABIDOPSIS-THALIANA ; CAROTENOID BIOSYNTHESIS ; FLAVONOID BIOSYNTHESIS ; DEVELOPING SEEDS ; SSR-MARKERS ; RED FLOWERS ; WEB SERVER ; RT-PCR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000370591700001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/25971]  |
| 专题 | 昆明植物研究所_中国西南野生生物种质资源库 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species Southwest China, Plant Germplasm & Genom Ctr, Kunming, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao, Qiu-Yang,Huang, Hui,Tong, Yan,et al. Transcriptome Analysis Identifies Candidate Genes Related to Triacylglycerol and Pigment Biosynthesis and Photoperiodic Flowering in the Ornamental and Oil-Producing Plant, Camellia reticulata (Theaceae)[J]. FRONTIERS IN PLANT SCIENCE,2016,7. |
| APA | Yao, Qiu-Yang,Huang, Hui,Tong, Yan,Xia, En-Hua,&Gao, Li-Zhi.(2016).Transcriptome Analysis Identifies Candidate Genes Related to Triacylglycerol and Pigment Biosynthesis and Photoperiodic Flowering in the Ornamental and Oil-Producing Plant, Camellia reticulata (Theaceae).FRONTIERS IN PLANT SCIENCE,7. |
| MLA | Yao, Qiu-Yang,et al."Transcriptome Analysis Identifies Candidate Genes Related to Triacylglycerol and Pigment Biosynthesis and Photoperiodic Flowering in the Ornamental and Oil-Producing Plant, Camellia reticulata (Theaceae)".FRONTIERS IN PLANT SCIENCE 7(2016). |
入库方式： OAI收割
来源：昆明植物研究所
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