In depth understanding the molecular response to the enhanced secretion of fatty acids in Saccharomyces cerevisiae due to one-step gene deletion of acyl-CoA synthetases
文献类型：期刊论文
| 作者 | Fang, Fang1; Dai, Bingye2; Zhao, Guang1 ; Zhao, Hua3; Sun, Chao1; Liu, Hui1; Xian, Mo1
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| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2016-09-01 |
| 卷号 | 51期号:9页码:1162-1174 |
| 关键词 | Fatty acids Cell envelope Biobased chemicals Integrated omics analysis Cellular response Saccharomyces cerevisiae |
| 英文摘要 | Considering of the possible application of fatty acids secretion in industrial production process for reducing extraction costs, the effects of enhanced fatty acids secretion by knocking out acyl-CoA synthetases on the global regulation, cellular metabolism were investigated through integrated omics analysis. Firstly, functional category analysis of differential expression genes showed that most of the genes were associated with the cell cycle and biology process such as cellular component biogenesis, cell wall organization and so on. Secondly, the decreased mRNA levels of Tdh, Eno and Pgk1 in glycolysis in faa mutant YB526 were confirmed by the differential protein analysis. As described in previous reports, the decreased protein levels of Tdh, Eno and Pgk1 were resulted from the increase of 14-3-3 protein activity. However, the increased level of 14-3-3 proteins could not improve the biosynthesis of ergosterol in the acyl-CoA deficiency strain YB526 due to the toxic effects of fatty acids. Thirdly, the decrease of ergosterol and ceramide contents in cell envelopes can improve the permeation of membrane and fatty acids secretion in faa mutant YB526. This comprehensive omics analysis could be helpful in the rational design of robust yeasts for the production of free fatty acids or other biobased chemicals. (C) 2016 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | SP PCC 6803 ; ESCHERICHIA-COLI ; BACILLUS-SUBTILIS ; INTEGRATIVE ANALYSIS ; PROTEOME ANALYSIS ; ANALYSIS REVEALS ; YEAST ; TRANSCRIPTOME ; METABOLISM ; RESISTANCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000382349500007 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/8489]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China 2.China Rural Technol Dev Ctr, Beijing 100045, Peoples R China 3.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, Fang,Dai, Bingye,Zhao, Guang,et al. In depth understanding the molecular response to the enhanced secretion of fatty acids in Saccharomyces cerevisiae due to one-step gene deletion of acyl-CoA synthetases[J]. PROCESS BIOCHEMISTRY,2016,51(9):1162-1174. |
| APA | Fang, Fang.,Dai, Bingye.,Zhao, Guang.,Zhao, Hua.,Sun, Chao.,...&Xian, Mo.(2016).In depth understanding the molecular response to the enhanced secretion of fatty acids in Saccharomyces cerevisiae due to one-step gene deletion of acyl-CoA synthetases.PROCESS BIOCHEMISTRY,51(9),1162-1174. |
| MLA | Fang, Fang,et al."In depth understanding the molecular response to the enhanced secretion of fatty acids in Saccharomyces cerevisiae due to one-step gene deletion of acyl-CoA synthetases".PROCESS BIOCHEMISTRY 51.9(2016):1162-1174. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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