Cellulose Chain Binding Free Energy Drives the Processive Move of Cellulases on the Cellulose Surface
文献类型：期刊论文
| 作者 | Wang, Yefei1,2; Zhang, Shujun1,2; Song, Xiangfei1,2; Yao, Lishan1,2 |
| 刊名 | BIOTECHNOLOGY AND BIOENGINEERING
 |
| 出版日期 | 2016-09-01 |
| 卷号 | 113期号:9页码:1873-1880 |
| 关键词 | Cel7B Trichoderma reesei processivity free energy calculations |
| 英文摘要 | Processivity is essential for cellulases in their catalysis of cellulose hydrolysis. But what drives the processive move is not well understood. In this work, we use Trichoderma reesei Cel7B as a model system and show that its processivity is directly correlated to the binding free energy difference of a cellulose chain occupying the binding sites -7 to +2 and that occupying sites -7 to -1. Several mutants that have stronger interactions with glycosyl units in sites +1 and +2 than the wild type enzyme show higher processivity. The results suggest that after the release of the product cellobiose located in sites +1 and +2, the enzyme pulls the cellulose chain to fill the vacant sites, which propels its processive move on the cellulose surface. (C) 2016 Wiley Periodicals, Inc. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | TRICHODERMA-REESEI CELLOBIOHYDROLASES ; AROMATIC-CARBOHYDRATE INTERACTIONS ; PARTICLE MESH EWALD ; MOLECULAR SIMULATION ; CRYSTALLINE CELLULOSE ; GLYCOSIDE HYDROLASES ; AUTOMATED DOCKING ; STRUCTURAL BASIS ; ENDOGLUCANASE-I ; ENZYMES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000384751500004 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/8479]  |
| 专题 | 青岛生物能源与过程研究所_仿真模拟团队 |
| 作者单位 | 1.Chinese Acad Sci, Shandong Prov Key Lab Synthet Biol, Qingdao, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biofuels, Qingdao 266061, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yefei,Zhang, Shujun,Song, Xiangfei,et al. Cellulose Chain Binding Free Energy Drives the Processive Move of Cellulases on the Cellulose Surface[J]. BIOTECHNOLOGY AND BIOENGINEERING,2016,113(9):1873-1880. |
| APA | Wang, Yefei,Zhang, Shujun,Song, Xiangfei,&Yao, Lishan.(2016).Cellulose Chain Binding Free Energy Drives the Processive Move of Cellulases on the Cellulose Surface.BIOTECHNOLOGY AND BIOENGINEERING,113(9),1873-1880. |
| MLA | Wang, Yefei,et al."Cellulose Chain Binding Free Energy Drives the Processive Move of Cellulases on the Cellulose Surface".BIOTECHNOLOGY AND BIOENGINEERING 113.9(2016):1873-1880. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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