Characterization of a Unique Pathway for 4-Cresol Catabolism Initiated by Phosphorylation in Corynebacterium glutamicum
文献类型：期刊论文
| 作者 | Du, Lei1,3; Ma, Li1 ; Qi, Feifei1; Zheng, Xianliang1,3; Jiang, Chengying4,5; Li, Ailei2; Wan, Xiaobo2; Liu, Shuang-Jiang4,5; Li, Shengying1
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| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
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| 出版日期 | 2016-03-18 |
| 卷号 | 291期号:12页码:6583-6594 |
| 关键词 | biodegradation cytochrome P450 gram-positive bacteria microbiology phosphorylation 4-cresol Corynebacterium glutamicum |
| 英文摘要 | 4-Cresol is not only a significant synthetic intermediate for production of many aromatic chemicals, but also a priority environmental pollutant because of its toxicity to higher organisms. In our previous studies, a gene cluster implicated to be involved in 4-cresol catabolism, creCDEFGHIR, was identified in Corynebacterium glutamicum and partially characterized in vivo. In this work, we report on the discovery of a novel 4-cresol biodegradation pathway that employs phosphorylated intermediates. This unique pathway initiates with the phosphorylation of the hydroxyl group of 4-cresol, which is catalyzed by a novel 4-methylbenzyl phosphate synthase, CreHI. Next, a unique class I P450 system, CreJEF, specifically recognizes phosphorylated intermediates and successively oxidizes the aromatic methyl group into carboxylic acid functionality via alcohol and aldehyde intermediates. Moreover, CreD (phosphohydrolase), CreC (alcohol dehydrogenase), and CreG (aldehyde dehydrogenase) were also found to be required for efficient oxidative transformations in this pathway. Steady-state kinetic parameters (K-m and k(cat)) for each catabolic step were determined, and these results suggest that kinetic controls serve a key role in directing the metabolic flux to the most energy effective route. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | P-CRESOL METHYLHYDROXYLASE ; BETA-KETOADIPATE PATHWAY ; PSEUDOMONAS-PUTIDA ; GEOBACTER-METALLIREDUCENS ; THAUERA-AROMATICA ; TRANSCRIPTIONAL REGULATION ; GENETIC-CHARACTERIZATION ; ANAEROBIC DEGRADATION ; PHENOL METABOLISM ; ENZYMES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000372894200043 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/8242]  |
| 专题 | 青岛生物能源与过程研究所_酶工程团队 |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Biofuels, Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Shandong, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Biobased Mat, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, State Key Lab Microbial Resources, Inst Microbiol, Beijing 100101, Peoples R China 5.Chinese Acad Sci, Environm Microbiol & Biotechnol Res Ctr, Inst Microbiol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Lei,Ma, Li,Qi, Feifei,et al. Characterization of a Unique Pathway for 4-Cresol Catabolism Initiated by Phosphorylation in Corynebacterium glutamicum[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2016,291(12):6583-6594. |
| APA | Du, Lei.,Ma, Li.,Qi, Feifei.,Zheng, Xianliang.,Jiang, Chengying.,...&Li, Shengying.(2016).Characterization of a Unique Pathway for 4-Cresol Catabolism Initiated by Phosphorylation in Corynebacterium glutamicum.JOURNAL OF BIOLOGICAL CHEMISTRY,291(12),6583-6594. |
| MLA | Du, Lei,et al."Characterization of a Unique Pathway for 4-Cresol Catabolism Initiated by Phosphorylation in Corynebacterium glutamicum".JOURNAL OF BIOLOGICAL CHEMISTRY 291.12(2016):6583-6594. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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