Aptamer and PNIPAAm co-conjugated nanoparticles regulate activity of enzyme with different temperature
文献类型：期刊论文
| 作者 | Yu, Jiemiao1,2,3; Yang, Liangrong2; Liang, Xiangfeng1 ; Dong, Tingting2,3; Qu, Hongnan1,2,3; Rong, Meng2,3; Liu, Huizhou2,3
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| 刊名 | TALANTA
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| 出版日期 | 2016-10-01 |
| 卷号 | 159页码:47-54 |
| 关键词 | Temperature responsive Gold nanoparticles Aptamer Thrombin Regulation Activity |
| 英文摘要 | In this paper, we described a temperature responsive nano-system that can regulate activity of enzyme with different temperature. Temperature responsive polymer poly(N-isopropylacrylamide) (PNIPAAm), with low critical solution temperature of 32 degrees C, was synthesized with thiol modification. PNIPAAm and thrombin aptamer were co-functionalized on the surface of gold nanoparticles for effective regulation of thrombin activity with different temperature. On the one hand, we studied the thermal responsive properties of this inhibitor via UV-visible spectroscopy. On the other hand, we investigated the regulation of thrombin activity by this platform with different temperature. The PNIPAAm chains could extend and shrink with different temperature, which suggested that PNIPAAm on the surface of gold nanoparticles could regulate interaction between thrombin and aptamer according to temperature changing. At 25 degrees C, PNIPAAm was hydrophilic extended state, which blocked the interaction between thrombin and aptamer on the surface of gold nanoparticles, therefore thrombin activity had no change. On the contrary, at 37 degrees C, PNIPAAm transformed from hydrophilic extended state to hydrophobic shrank state, allowing the aptamer to capture thrombin, inhibiting the activity of thrombin. More interestingly, this regulation was reverse to normal condition, where 37 degrees C was always the optimum reaction temperature for most of human enzymes. This system we prepared was opposite, which was capable of inhibiting the thrombin activity at 37 degrees C. Furthermore, this was the first report of regulation of thrombin activity using this temperature responsive platform. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | GOLD NANOPARTICLES ; IN-VIVO ; N-ISOPROPYLACRYLAMIDE ; LIQUID-CHROMATOGRAPHY ; THROMBIN DETECTION ; DNA ; INHIBITION ; APTASENSOR ; BIND ; COAGULATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000381322000008 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/8496]  |
| 专题 | 资源环境与绿色分离团队 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Jiemiao,Yang, Liangrong,Liang, Xiangfeng,et al. Aptamer and PNIPAAm co-conjugated nanoparticles regulate activity of enzyme with different temperature[J]. TALANTA,2016,159:47-54. |
| APA | Yu, Jiemiao.,Yang, Liangrong.,Liang, Xiangfeng.,Dong, Tingting.,Qu, Hongnan.,...&Liu, Huizhou.(2016).Aptamer and PNIPAAm co-conjugated nanoparticles regulate activity of enzyme with different temperature.TALANTA,159,47-54. |
| MLA | Yu, Jiemiao,et al."Aptamer and PNIPAAm co-conjugated nanoparticles regulate activity of enzyme with different temperature".TALANTA 159(2016):47-54. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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