Exploring the Synergy between Cellobiose Dehydrogenase from Phanerochaete chrysosporium and Cellulase from Trichoderma reesei
文献类型：期刊论文
| 作者 | Wang, Min1,2; Lu, Xuefeng1,3 |
| 刊名 | FRONTIERS IN MICROBIOLOGY
 |
| 出版日期 | 2016-04-29 |
| 卷号 | 7 |
| 关键词 | Tritroderma reesei cellulase cellobiose inhibition cellobiose dehydrogenase synergy |
| 英文摘要 | Recent demands for the production of lignocellulose biofuels boosted research on cellulase. Hydrolysis efficiency and production cost of cellulase are two bottlenecks in "biomass to biofuels" process. The Trichoderma cellulase mixture is one of the most commonly used enzymes for cellulosic hydrolysis. During hydrolytic process cellobiose accumulation causes feedback inhibition against most cellobiohydrolases and endoglucanases. In this study, we demonstrated the synergism effects between cellobiose dehydrogenase (CDH) and cellulase both in vitro and in vivo. The CDH from Phanerochaete chrysosporium was heterologously expressed in Pichia pastoris. Supplementation of the purified CDH in Trichoderma cellulase increased the cellulase activities. Especially beta-glucosidase activity was increased by 30-100% varying at different time points. On the other hand, the cdh gene was heterologously expressed in Trichoderma reesei to explore the synergism between CDH and cellulases in vivo. The analyses of gene expression and enzymatic profiles of filter paper activity, carboxymethylcellulase (CMCase) and beta-glucosidase show the increased cellulase activity and the enhanced cellulase production in the cdh-expressing strains. The results elucidate a possible mechanism for diminishing the cellobiose inhibition of cellulase by CDH. These findings provide a novel perspective to make more economic enzyme cocktails for commercial application or explore alternative strategies for generating cellulase-producing strains with higher efficiency. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | HYPOCREA-JECORINA ; BETA-GLUCOSIDASE ; ENZYMATIC-HYDROLYSIS ; GENE ; CELLOBIOHYDROLASE ; DEGRADATION ; EXPRESSION ; INHIBITION ; INDUCTION ; BIOFUELS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000375388100001 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/8297]  |
| 专题 | 青岛生物能源与过程研究所_微生物代谢工程团队 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Provinc Key Lab Energy Genet, Qingdao, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Provinc Key Lab Synthet Biol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Min,Lu, Xuefeng. Exploring the Synergy between Cellobiose Dehydrogenase from Phanerochaete chrysosporium and Cellulase from Trichoderma reesei[J]. FRONTIERS IN MICROBIOLOGY,2016,7. |
| APA | Wang, Min,&Lu, Xuefeng.(2016).Exploring the Synergy between Cellobiose Dehydrogenase from Phanerochaete chrysosporium and Cellulase from Trichoderma reesei.FRONTIERS IN MICROBIOLOGY,7. |
| MLA | Wang, Min,et al."Exploring the Synergy between Cellobiose Dehydrogenase from Phanerochaete chrysosporium and Cellulase from Trichoderma reesei".FRONTIERS IN MICROBIOLOGY 7(2016). |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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