The potential of Synechococcus elongatus UTEX 2973 for sugar feedstock production
文献类型：期刊论文
| 作者 | Song, Kuo1,2; Tan, Xiaoming1; Liang, Yajing1; Lu, Xuefeng1 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2016-09-01 |
| 卷号 | 100期号:18页码:7865-7875 |
| 关键词 | Glycogen Sucrose Salt stress Synechococcus elongatus UTEX 2973 |
| 英文摘要 | It is important to obtain abundant sugar feedstocks economically and sustainably for bio-fermentation industry, especially for producing cheap biofuels and biochemicals. Besides plant biomass, photosynthetic cyanobacteria have also been considered to be potential microbe candidates for sustainable production of carbohydrate feedstocks. As the fastest growing cyanobacterium reported so far, Synechococcus elongatus UTEX 2973 (Syn2973) might have huge potential for bioproduction. In this study, we explored the potentials of this strain as photo-bioreactors for sucrose and glycogen production. Under nitrogen-replete condition, Syn2973 could accumulate glycogen with a rate of 0.75 g L-1 day(-1) at the exponential phase and reach a glycogen content as high as 51 % of the dry cell weight (DCW) at the stationary phase. By introducing a sucrose transporter CscB, Syn2973 was endowed with an ability to secrete over 94 % sucrose out of cells under salt stress condition. The highest extracellular sucrose productivity reached 35.5 mg L-1 h(-1) for the Syn2973 strain expressing cscB, which contained the similar amounts of intracellular glycogen with the wild type. Potassium chloride was firstly proved to induce sucrose accumulation as well as sodium chloride in Syn2973. By semi-continuous culturing, 8.7 g L-1 sucrose was produced by the cscB-expressing strain of Syn2973 in 21 days. These results support that Syn2973 is a promising candidate with great potential for production of sugars. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | STRAIN PCC 7002 ; GLYCOGEN-SYNTHESIS ; CYANOBACTERIA ; NITROGEN ; SALT ; ACCLIMATION ; CONVERSION ; BIOFUELS ; SUCROSE ; ETHANOL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000382008000007 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/8421]  |
| 专题 | 青岛生物能源与过程研究所_微生物代谢工程团队 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Shandong Prov Key Lab Synthet Biol, 189 Songling Rd, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Kuo,Tan, Xiaoming,Liang, Yajing,et al. The potential of Synechococcus elongatus UTEX 2973 for sugar feedstock production[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2016,100(18):7865-7875. |
| APA | Song, Kuo,Tan, Xiaoming,Liang, Yajing,&Lu, Xuefeng.(2016).The potential of Synechococcus elongatus UTEX 2973 for sugar feedstock production.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,100(18),7865-7875. |
| MLA | Song, Kuo,et al."The potential of Synechococcus elongatus UTEX 2973 for sugar feedstock production".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 100.18(2016):7865-7875. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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