A one-step bioprocess for production of high-content fructo-oligosaccharides from inulin by yeast
文献类型：期刊论文
| 作者 | Wang, Da1,3; Li, Fu-Li1,2 ; Wang, Shi-An1
|
| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2016-10-20 |
| 卷号 | 151页码:1220-1226 |
| 关键词 | Fructo-oligosaccharides Inulin One-step bioprocess Yeast |
| 英文摘要 | Commercial fructo-oligosaccharides (FOS) are predominantly produced from sucrose by transfructosylation process that presents a maximum theoretical yield below 0.60 g(Fos) g(Sucrose)(-1). To obtain high-content FOS, costly purification is generally employed. Additionally, high-content FOS can be produced from inulin by using endo-inulinases. However, commercial endo-inulinases have not been extensively used in scale-up production of FOS. In the present study, a one-step bioprocess that integrated endo-inulinase production, FOS fermentation, and non-FOS sugars removal into one reactor was proposed to produce high-content FOS from inulin. The bioprocess was implemented by a recombinant yeast strain JZH Delta S-TSC, in which a heterologous endo-inulinase gene was expressed and the inherent invertase gene SUC2 was disrupted. FOS fermentation at 40 degrees C from 200 g/L chicory inulin presented the maximun titer, yield, and productivity of 180.2 +/- 0.8 g/L, 0.9 g(Fos) g(Inulin)(-1), and 7.51 +/- 0.03 g/L/h, respectively. This study demonstrated that the one-step bioprocess was simple and highly efficient. (C) 2016 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
| 研究领域[WOS] | Chemistry ; Polymer Science |
| 关键词[WOS] | INULOOLIGOSACCHARIDE PRODUCTION ; ASPERGILLUS-FICUUM ; ENDOINULINASE ; FRUCTOOLIGOSACCHARIDES ; PURIFICATION ; GENE ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000380943900137 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/8433]  |
| 专题 | 青岛生物能源与过程研究所_微生物资源团队 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Da,Li, Fu-Li,Wang, Shi-An. A one-step bioprocess for production of high-content fructo-oligosaccharides from inulin by yeast[J]. CARBOHYDRATE POLYMERS,2016,151:1220-1226. |
| APA | Wang, Da,Li, Fu-Li,&Wang, Shi-An.(2016).A one-step bioprocess for production of high-content fructo-oligosaccharides from inulin by yeast.CARBOHYDRATE POLYMERS,151,1220-1226. |
| MLA | Wang, Da,et al."A one-step bioprocess for production of high-content fructo-oligosaccharides from inulin by yeast".CARBOHYDRATE POLYMERS 151(2016):1220-1226. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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