Putative methyltransferase LaeA and transcription factor CreA are necessary for proper asexual development and controlling secondary metabolic gene cluster expression
文献类型：期刊论文
| 作者 | Zhang, Xiujun1,2,3; Zhu, Yingying1,2; Bao, Longfei1,2; Gao, Liwei1,2; Yao, Guangshan1,2; Li, Yanan1,2,3; Yang, Zhifeng4; Li, Zhonghai1,2; Zhong, Yaohua1,2; Li, Fuli5
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| 刊名 | FUNGAL GENETICS AND BIOLOGY
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| 出版日期 | 2016-09-01 |
| 卷号 | 94页码:32-46 |
| 关键词 | Penicillium oxalicum LaeA CreA Asexual development Secondary metabolism |
| 英文摘要 | The morphological development of fungi is a complex process and is often coupled with secondary metabolite production. In this study, we assessed the function of putative methyltransferase LaeA and transcription factor CreA in controlling asexual development and secondary metabolic gene cluster expression in Penicillium oxalicum. The deletion of laeA (Delta laeA) impaired the conidiation in P. oxalicum, with a downregulated expression of brlA. Overexpression of P. oxalicum brlA in Delta laeA could upregulate brlA and abaA remarkably, but could not rescue the conidiation defect; therefore, brlA and abaA expression were necessary but not sufficient for conidiation. Deletion of creA in Delta laeA background (Delta laeA Delta creA) blocked conidiation with a white fluffy phenotype. Nutrient-rich medium could not rescue developmental defects in Delta laeA Delta creA mutant but could rescue defects in Delta laeA. Expression of 10 genes, namely, albA/wA, abrB/yA, arpA, aygA, arpA-like, arpB, arpB-like, rodA, rodA-like, and rodB, for pigmentation and spore wall protein genes was silenced in Delta laeA Delta creA, whereas only six of them were downregulated in Delta laeA. Among the 28 secondary metabolism gene clusters in P. oxalicum, four secondary metabolism gene clusters were silenced in Delta laeA and two were also silenced in Delta brlA mutant. A total of 10 physically linked and coregulated genes were distributed over five chromosomes in Delta laeA. Six of these genes were located in subtelomeric regions, thus demonstrating a positional bias for LaeA-regulated clusters toward subtelomeric regions. All of silenced clusters located in subtelomeric regions were derepressed in Delta laeA Delta creA, hence showing that lack of CreA could remediate the repression of gene clusters in Delta laeA background. Results show that both putative methyltransferase LaeA and transcription factor CreA are necessary for proper asexual development and controlling secondary metabolic gene cluster expression. (C) 2016 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Genetics & Heredity ; Mycology |
| 研究领域[WOS] | Genetics & Heredity ; Mycology |
| 关键词[WOS] | CARBON CATABOLITE REPRESSION ; ASPERGILLUS-NIDULANS ; PENICILLIUM-CHRYSOGENUM ; TRICHODERMA-REESEI ; FILAMENTOUS FUNGI ; CONIDIOPHORE DEVELOPMENT ; CHEMICAL DIVERSITY ; ROQUEFORTINE C ; BIOSYNTHESIS ; FUMIGATUS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000381242900005 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/8500]  |
| 专题 | 青岛生物能源与过程研究所_微生物资源团队 |
| 作者单位 | 1.Shandong Univ, Natl Glycoengn Res Ctr, Jinan 250100, Peoples R China 2.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China 3.Shandong Univ, Shandong Prov Key Lab Carbohydrate Chem & Glycobi, Jinan 250100, Peoples R China 4.Shandong Univ, Sch Math, Jinan 250100, Peoples R China 5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 6.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xiujun,Zhu, Yingying,Bao, Longfei,et al. Putative methyltransferase LaeA and transcription factor CreA are necessary for proper asexual development and controlling secondary metabolic gene cluster expression[J]. FUNGAL GENETICS AND BIOLOGY,2016,94:32-46. |
| APA | Zhang, Xiujun.,Zhu, Yingying.,Bao, Longfei.,Gao, Liwei.,Yao, Guangshan.,...&Qin, Yuqi.(2016).Putative methyltransferase LaeA and transcription factor CreA are necessary for proper asexual development and controlling secondary metabolic gene cluster expression.FUNGAL GENETICS AND BIOLOGY,94,32-46. |
| MLA | Zhang, Xiujun,et al."Putative methyltransferase LaeA and transcription factor CreA are necessary for proper asexual development and controlling secondary metabolic gene cluster expression".FUNGAL GENETICS AND BIOLOGY 94(2016):32-46. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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