The Miscanthus NAC transcription factor MlNAC9 enhances abiotic stress tolerance in transgenic Arabidopsis
文献类型：期刊论文
| 作者 | Zhao, Xun1,2; Yang, Xuanwen1; Pei, Shengqiang1,2; He, Guo1 ; Wang, Xiaoyu1,2; Tang, Qi1; Jia, Chunlin3; Lu, Ying4; Hu, Ruibo1; Zhou, Gongke1
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| 刊名 | GENE
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| 出版日期 | 2016-07-15 |
| 卷号 | 586期号:1页码:158-169 |
| 关键词 | Miscanthus lutarioriparius Abiotic stress NAC transcription factor Stress tolerance |
| 英文摘要 | NAC (NAM, ATAF1/2, and CUC2) transcription factors are known to play important roles in responses to abiotic stresses in plants. Currently, little information regarding the functional roles of NAC genes in stress tolerance is available in Miscanthus lutarioriparius, a promising bioenergy plant for cellulosic ethanol production. In this study, we carried out the functional characterization of MlNAC9 in abiotic stresses. MlNAC9 was shown to act as a nuclear localized transcription activator with the activation domain in its C-terminus. The overexpression of MlNAC9 in Arabidopsis conferred hypersensitivity to abscisic acid (ABA) at seed germination and root elongation stages. In addition, the overexpression of MlNAC9 led to increased seed germination rate and root growth under salt (NaCl) treatment. Meanwhile, the transgenic Arabidopsis overexpressing MlNAC9 showed enhanced tolerance to drought and cold stresses. The expression of stress-responsive marker genes was significantly increased in MlNAC9 overexpression lines compared to that of WT under ABA, drought, salt, and cold stresses. Correspondingly, the activities of antioxidant enzymes superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were significantly increased and the malondialdehyde (MDA) content was lower accumulated in MlNAC9 overexpression lines under drought and salt treatments. These results indicated that the overexpression of MlNAC9 improved the tolerance to abiotic stresses via an ABA-dependent pathway, and the enhanced tolerance of transgenic plants was mainly attributed to the increased expression of stress-responsive genes and the enhanced scavenging capability of reactive oxygen species (ROS). (C) 2016 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Genetics & Heredity |
| 研究领域[WOS] | Genetics & Heredity |
| 关键词[WOS] | SIGNAL-TRANSDUCTION ; FUNCTIONAL-ANALYSIS ; DROUGHT TOLERANCE ; SALT TOLERANCE ; GENE ; COLD ; DEHYDRATION ; PLANTS ; WHEAT ; RICE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000376696000020 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/8271]  |
| 专题 | 青岛生物能源与过程研究所_植物代谢工程团队 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Res Ctr Biomass Resources & Environm, Key Lab Biofuels, Qingdao 265101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Shandong Inst Agr Sustainable Dev, Jinan 250100, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Natl Ctr Gene Res, Shanghai 200233, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xun,Yang, Xuanwen,Pei, Shengqiang,et al. The Miscanthus NAC transcription factor MlNAC9 enhances abiotic stress tolerance in transgenic Arabidopsis[J]. GENE,2016,586(1):158-169. |
| APA | Zhao, Xun.,Yang, Xuanwen.,Pei, Shengqiang.,He, Guo.,Wang, Xiaoyu.,...&Zhou, Gongke.(2016).The Miscanthus NAC transcription factor MlNAC9 enhances abiotic stress tolerance in transgenic Arabidopsis.GENE,586(1),158-169. |
| MLA | Zhao, Xun,et al."The Miscanthus NAC transcription factor MlNAC9 enhances abiotic stress tolerance in transgenic Arabidopsis".GENE 586.1(2016):158-169. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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