Structural and functional characterization of ochratoxinase, a novel mycotoxin-degrading enzyme
文献类型：期刊论文
| 作者 | Dobritzsch, Doreen1,2; Wang, Huaming3,4; Schneider, Gunter1; Yu, Shukun5,6 |
| 刊名 | BIOCHEMICAL JOURNAL
 |
| 出版日期 | 2014-09-15 |
| 卷号 | 462页码:441-452 |
| 关键词 | metal-dependent amidohydrolase mycotoxin ochratoxin degradation protein crystal structure |
| 英文摘要 | Ochratoxin, with ochratoxin A as the dominant form, is one of the five major mycotoxins most harmful to humans and animals. It is produced by Aspergillus and Penicillium species and occurs in a wide range of agricultural products. Detoxification of contaminated food is a challenging health issue. In the present paper we report the identification, characterization and crystal structure (at 2.2 angstrom) of a novel microbial ochratoxinase from Aspergillus niger. A putative amidase gene encoding a 480 amino acid polypeptide was cloned and homologously expressed in A. niger. The recombinant protein is N-terminally truncated, thermostable, has optimal activity at pH similar to 6 and 66 degrees C, and is more efficient in ochratoxin A hydrolysis than carboxypeptidase A and Y, the two previously known enzymes capable of degrading this mycotoxin. The subunit of the homo-octameric enzyme folds into a two-domain structure characteristic of a metal dependent amidohydrolase, with a twisted TIM (triosephosphateisomerase)-barrel and a smaller beta-sandwich domain. The active site contains an aspartate residue for acid base catalysis, and a carboxylated lysine and four histidine residues for binding of a binuclear metal centre. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | AMIDOHYDROLASE SUPERFAMILY ; ASPERGILLUS-NIGER ; STRUCTURE VALIDATION ; DEGRADATION ; HYDROLYSIS ; GENE ; FOOD ; BIODEGRADATION ; IDENTIFICATION ; DETOXIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000341802100006 |
| 源URL | [http://124.16.173.210/handle/834782/1332]  |
| 专题 | 天津工业生物技术研究所_蛋白质表达及分子生物学实验室 王华明 _期刊论文 |
| 作者单位 | 1.Karolinska Inst, Dept Med Biochem & Biophys, SE-17177 Stockholm, Sweden 2.Uppsala Univ, Dept Chem, BMC, SE-75123 Uppsala, Sweden 3.Danisco AS DuPont, Genencor, Palo Alto, CA 94304 USA 4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 5.Danisco AS DuPont, Genencor, DK-8220 Brabrand, Denmark 6.Lund Univ, Dept Biotechnol, SE-22100 Lund, Sweden |
| 推荐引用方式 GB/T 7714 | Dobritzsch, Doreen,Wang, Huaming,Schneider, Gunter,et al. Structural and functional characterization of ochratoxinase, a novel mycotoxin-degrading enzyme[J]. BIOCHEMICAL JOURNAL,2014,462:441-452. |
| APA | Dobritzsch, Doreen,Wang, Huaming,Schneider, Gunter,&Yu, Shukun.(2014).Structural and functional characterization of ochratoxinase, a novel mycotoxin-degrading enzyme.BIOCHEMICAL JOURNAL,462,441-452. |
| MLA | Dobritzsch, Doreen,et al."Structural and functional characterization of ochratoxinase, a novel mycotoxin-degrading enzyme".BIOCHEMICAL JOURNAL 462(2014):441-452. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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