Structure of the Fusion Core and Inhibition of Fusion by a Heptad Repeat Peptide Derived from the S Protein of Middle East Respiratory Syndrome Coronavirus
文献类型：期刊论文
| 作者 | Gao, Jing1,2; Lu, Guangwen1; Qi, Jianxun1; Li, Yan1; Wu, Ying1; Deng, Yao3; Geng, Heyuan3; Li, Hongbin4; Wang, Qihui1; Xiao, Haixia5 |
| 刊名 | JOURNAL OF VIROLOGY
 |
| 出版日期 | 2013-12-01 |
| 卷号 | 87期号:24页码:13134-13140 |
| 英文摘要 | Middle East respiratory syndrome coronavirus (MERS-CoV) recently emerged as a severe worldwide public health concern. The virus is highly pathogenic, manifesting in infected patients with an approximately 50% fatality rate. It is known that the surface spike (S) proteins of coronaviruses mediate receptor recognition and membrane fusion, thereby playing an indispensable role in initiating infection. In this process, heptad repeats 1 and 2 (HR1 and HR2) of the S protein assemble into a complex called the fusion core, which represents a key membrane fusion architecture. To date, however, the MERS-CoV fusion core remains un-characterized. In this study, we performed a series of biochemical and biophysical analyses characterizing the HR1/HR2 complexes of this novel virus. The HR sequences were variably truncated and then connected with a flexible amino acid linker. In each case, the recombinant protein automatically assembled into a trimer in solution, displaying a typical alpha-helical structure. One of these trimers was successfully crystallized, and its structure was solved at a resolution of 1.9 angstrom. A canonical 6-helix bundle, like those reported for other coronaviruses, was revealed, with three HR1 helices forming the central coiled-coil core and three HR2 chains surrounding the core in the HR1 side grooves. This demonstrates that MERS-CoV utilizes a mechanism similar to those of other class I enveloped viruses for membrane fusion. With this notion, we further identified an HR2-based peptide that could potently inhibit MERS-CoV fusion and entry by using a pseudotyped-virus system. These results lay the groundwork for future inhibitory peptidic drug design. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Virology |
| 研究领域[WOS] | Virology |
| 关键词[WOS] | VIRAL MEMBRANE-FUSION ; SPIKE PROTEIN ; NEUTRALIZING ANTIBODIES ; CRYSTAL-STRUCTURE ; COILED-COIL ; VIRUS ; ENTRY ; RECEPTOR ; COV ; INFECTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000327443300006 |
| 源URL | [http://124.16.173.210/handle/834782/1282]  |
| 专题 | 天津工业生物技术研究所_蛋白质工程与疫苗实验室 高福_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Ctr Dis Control & Prevent China CDC, Inst Viral Dis Control & Prevent, Biotech Ctr Viral Dis Emergency, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Microbiol, Core Facil, Beijing, Peoples R China 5.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Lab Prot Engn & Vaccines, Tianjin, Peoples R China 6.Chinese Acad Sci, Beijing Inst Life Sci, RNIH, Beijing, Peoples R China 7.Chinese Ctr Dis Control & Prevent China CDC, Off Director Gen, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Jing,Lu, Guangwen,Qi, Jianxun,et al. Structure of the Fusion Core and Inhibition of Fusion by a Heptad Repeat Peptide Derived from the S Protein of Middle East Respiratory Syndrome Coronavirus[J]. JOURNAL OF VIROLOGY,2013,87(24):13134-13140. |
| APA | Gao, Jing.,Lu, Guangwen.,Qi, Jianxun.,Li, Yan.,Wu, Ying.,...&Gao, George F..(2013).Structure of the Fusion Core and Inhibition of Fusion by a Heptad Repeat Peptide Derived from the S Protein of Middle East Respiratory Syndrome Coronavirus.JOURNAL OF VIROLOGY,87(24),13134-13140. |
| MLA | Gao, Jing,et al."Structure of the Fusion Core and Inhibition of Fusion by a Heptad Repeat Peptide Derived from the S Protein of Middle East Respiratory Syndrome Coronavirus".JOURNAL OF VIROLOGY 87.24(2013):13134-13140. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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