Development of beta-Amino Acid Dehydrogenase for the Synthesis of beta-Amino Acids via Reductive Amination of beta-Keto Acids
文献类型：期刊论文
| 作者 | Zhang, Dalong; Chen, Xi; Zhang, Rui; Yao, Peiyuan; Wu, Qiaqing; Zhu, Dunming1 |
| 刊名 | ACS CATALYSIS
 |
| 出版日期 | 2015-04-01 |
| 卷号 | 5期号:4页码:2220-2224 |
| 关键词 | asymmetric catalysis beta-amino acid dehydrogenase beta-amino acid biocatalysis reductive amination enzyme engineering |
| 英文摘要 | An L-erythro-3,5-diaminohexanoate dehydrogenase from Candidatus Cloacamonas acidaminovorans (3,5-DAHDHcca) was engineered to beta-amino acid dehydrogenase (beta-AADH) by screening a domain scanning mutagenesis library. The best beta-AADH mutant displayed about 200-fold activity toward (R)-beta-homomethionine compared with the wild-type enzyme. (R)-beta-Homomethionine, (R)-beta-phenylalanine, and (S)-beta-aminobutyric acid were obtained via beta-AADH-catalyzed reductive amination of the corresponding beta-keto acids, which were generated from beta-keto-nitriles or beta-keto-esters with nitrilase or lipase. This is the first example of the direct reductive amination of beta-keto acids catalyzed by beta-AADH to give beta-amino acids, opening a new venue for the synthesis of chiral beta-amino acids. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Physical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | NAD-DEPENDENT DEHYDROGENASES ; SITE-DIRECTED MUTAGENESIS ; ATOM TRANSFER-REACTIONS ; ENANTIOSELECTIVE SYNTHESIS ; BETA(2)-AMINO ACIDS ; LEUCINE DEHYDROGENASE ; GLUTAMATE-DEHYDROGENASE ; SUBSTRATE-SPECIFICITY ; ASYMMETRIC-SYNTHESIS ; OMEGA-TRANSAMINASES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000352464400025 |
| 源URL | [http://124.16.173.210/handle/834782/1452]  |
| 专题 | 天津工业生物技术研究所_发酵与生物化工工程 李正华_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Tianjin Engn Ctr Biocatalyt Technol, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Dalong,Chen, Xi,Zhang, Rui,et al. Development of beta-Amino Acid Dehydrogenase for the Synthesis of beta-Amino Acids via Reductive Amination of beta-Keto Acids[J]. ACS CATALYSIS,2015,5(4):2220-2224. |
| APA | Zhang, Dalong,Chen, Xi,Zhang, Rui,Yao, Peiyuan,Wu, Qiaqing,&Zhu, Dunming.(2015).Development of beta-Amino Acid Dehydrogenase for the Synthesis of beta-Amino Acids via Reductive Amination of beta-Keto Acids.ACS CATALYSIS,5(4),2220-2224. |
| MLA | Zhang, Dalong,et al."Development of beta-Amino Acid Dehydrogenase for the Synthesis of beta-Amino Acids via Reductive Amination of beta-Keto Acids".ACS CATALYSIS 5.4(2015):2220-2224. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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