Metabolic engineering of Escherichia coli and in silico comparing of carboxylation pathways for high succinate productivity under aerobic conditions
文献类型：期刊论文
| 作者 | Yang, Jiangang1,2,3,4; Wang, Zhiwen1,2; Zhu, Nianqing1,2; Wang, Baiyun1,2; Chen, Tao1,2; Zhao, Xueming1,2 |
| 刊名 | MICROBIOLOGICAL RESEARCH
 |
| 出版日期 | 2014 |
| 卷号 | 169期号:5-6页码:432-440 |
| 关键词 | Aerobic conditions In silico Escherichia coli Metabolic engineering Succinate |
| 英文摘要 | A novel aerobic succinate production system was strategically designed that allows Escherichia coli to produce and accumulate succinate with high specific productivity under aerobic conditions. Mutations in the tricarboxylic acid cycle (sdhA, icIR) and byproduct formation pathways (poxB, ackA-pta, mgsA) of E. coli were created to construct the glyoxylate cycle and oxidative branch of the TCA cycle for aerobic succinate production. Strain ZJG13 (Delta sdhA, Delta ackA-pta, Delta poxB, Delta mgsA, Delta icIR) exhibited normal growth behavior and accumulated succinate with an average specific productivity of 0.50 mmolg CDW-1 h(-1) during the fermentation. The glyoxylate shunt operon aceKBA was overexpressed by introducing plasmid pT9aceICAB to ZJG13; the resulting strain had minor effect on productivity improvement. To fully understand the effect of the carboxylation reactions on succinate production, three reactions catalyzed by pyruvate carboxylase (PYC), malic enzyme (MAEA) and phosphoenolpyruvate carboxylase (PPC) were analyzed by a Computational Approach for Strain Optimization aiming at high Productivity (CASOP). Based on the CASOP analysis, carboxylation reaction catalyzed by PYC was the most suitable one to obtain high productivity. When pyc was overexpressed in ZJG13, the specific succinate productivity further increased to 0.76 mmol g CDW-1 h(-1). Fed-batch culture of the strain ZJG13/pT184pyc led to a titer of 36.1 g/L succinate, with a specific productivity of 2.75 mmol g CDW-1 h(-1) which stands for the highest value among currently reported aerobic bacterial succinate producers. These results indicate that the CASOP strategy is useful as a guiding tool for the rational strain design with high productivity. (C) 2013 Elsevier GmbH. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | CORYNEBACTERIUM-GLUTAMICUM ; BIOCHEMICAL PATHWAYS ; ENERGY-PRODUCTION ; ACID PRODUCTION ; SUCCINICIPRODUCENS ; EVOLUTION ; NETWORKS ; STRAINS ; LACTATE ; GLUCOSE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000335107900014 |
| 源URL | [http://124.16.173.210/handle/834782/1329]  |
| 专题 | 天津工业生物技术研究所_功能糖与天然活性物质研究组 孙媛霞 _期刊论文 |
| 作者单位 | 1.Tianjin Univ, Minist Educ, Key Lab Syst Bioengn, Tianjin 300072, Peoples R China 2.Tianjin Univ, Sch Chem Engn & Technol, Dept Biochem Engn, Tianjin 300072, Peoples R China 3.Chinese Acad Sci, Natl Engn Lab Ind Enzymes, Tianjin, Peoples R China 4.Chinese Acad Sci, Tianjin Engn Ctr Biocatalyt Technol, Tianjin Inst Ind Biotechnol, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Jiangang,Wang, Zhiwen,Zhu, Nianqing,et al. Metabolic engineering of Escherichia coli and in silico comparing of carboxylation pathways for high succinate productivity under aerobic conditions[J]. MICROBIOLOGICAL RESEARCH,2014,169(5-6):432-440. |
| APA | Yang, Jiangang,Wang, Zhiwen,Zhu, Nianqing,Wang, Baiyun,Chen, Tao,&Zhao, Xueming.(2014).Metabolic engineering of Escherichia coli and in silico comparing of carboxylation pathways for high succinate productivity under aerobic conditions.MICROBIOLOGICAL RESEARCH,169(5-6),432-440. |
| MLA | Yang, Jiangang,et al."Metabolic engineering of Escherichia coli and in silico comparing of carboxylation pathways for high succinate productivity under aerobic conditions".MICROBIOLOGICAL RESEARCH 169.5-6(2014):432-440. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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