Enzymatic conversion of D-galactose to D-tagatose: Cloning, overexpression and characterization of L-arabinose isomerase from Pediococcus pentosaceus PC-5
文献类型：期刊论文
| 作者 | Men, Yan1; Zhu, Yueming1; Zhang, Lili1; Kang, Zhenkui2; Izumori, Ken1; Sun, Yuanxia1; Ma, Yanhe1 |
| 刊名 | MICROBIOLOGICAL RESEARCH
 |
| 出版日期 | 2014 |
| 卷号 | 169期号:2-3页码:171-178 |
| 关键词 | L-Arabinose isomerase D-Tagatose Food-grade microorganisms Pediococcus pentosaceus |
| 英文摘要 | The gene encoding L-arabinose isomerase from food-grade strain Pediococcus pentosaceus PC-5 was cloned and overexpressed in Escherichia coli. The recombinant protein was purified and characterized. It was optimally active at 50 degrees C and pH 6.0. Furthermore, this enzyme exhibited a weak requirement for metallic ions for its maximal activity evaluated at 0.6 mM Mn2+ or 0.8 mM Co2+. Interestingly, this enzyme was distinguished from other L-AIs, it could not use L-arabinose as its substrate. In addition, a three-dimensional structure of L.-AI was built by homology modeling and L-arabinose and D-galactose were docked into the active site pocket of PPAI model to explain the interaction between L-AI and its substrate. The purified P. pentosaceus PC-5 L-AI converted P-galactose into D-tagatose with a high conversion rate of 52% after 24 h at 50 degrees C, suggesting its excellent potential in D-tagatose production. Crown Copyright (C) 2013 Published by Elsevier GmbH. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | THERMAL-STABILITY ; GLUCOSE ISOMERASE ; PROTEIN-STRUCTURE ; ESCHERICHIA-COLI ; SWISS-MODEL ; PH OPTIMUM ; ACIDIC PH ; PURIFICATION ; EXPRESSION ; STRAIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000330335600008 |
| 源URL | [http://124.16.173.210/handle/834782/1409]  |
| 专题 | 天津工业生物技术研究所_功能糖与天然活性物质研究组 孙媛霞 _期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China 2.Shanxi Tianjiao Biol Co Ltd, Shanxin 030006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Men, Yan,Zhu, Yueming,Zhang, Lili,et al. Enzymatic conversion of D-galactose to D-tagatose: Cloning, overexpression and characterization of L-arabinose isomerase from Pediococcus pentosaceus PC-5[J]. MICROBIOLOGICAL RESEARCH,2014,169(2-3):171-178. |
| APA | Men, Yan.,Zhu, Yueming.,Zhang, Lili.,Kang, Zhenkui.,Izumori, Ken.,...&Ma, Yanhe.(2014).Enzymatic conversion of D-galactose to D-tagatose: Cloning, overexpression and characterization of L-arabinose isomerase from Pediococcus pentosaceus PC-5.MICROBIOLOGICAL RESEARCH,169(2-3),171-178. |
| MLA | Men, Yan,et al."Enzymatic conversion of D-galactose to D-tagatose: Cloning, overexpression and characterization of L-arabinose isomerase from Pediococcus pentosaceus PC-5".MICROBIOLOGICAL RESEARCH 169.2-3(2014):171-178. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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