Construction of allitol synthesis pathway by multi-enzyme coexpression in Escherichia coli and its application in allitol production
文献类型：期刊论文
| 作者 | Zhu, Yueming1; Li, Hongyi2; Liu, Pingping1; Yang, Jiangang1; Zhang, Xueli1; Sun, Yuanxia1 |
| 刊名 | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2015-05-01 |
| 卷号 | 42期号:5页码:661-669 |
| 关键词 | Allitol Multi-enzyme coupling pathway Cofactor recycling system Whole-cell biotransformation |
| 英文摘要 | An engineered strain for the conversion of d-fructose to allitol was developed by constructing a multi-enzyme coupling pathway and cofactor recycling system in Escherichia coli. d-Psicose-3-epimerase from Ruminococcus sp. and ribitol dehydrogenase from Klebsiella oxytoca were coexpressed to form the multi-enzyme coupling pathway for allitol production. The cofactor recycling system was constructed using the formate dehydrogenase gene from Candida methylica for continuous NADH supply. The recombinant strain produced 10.62 g/l allitol from 100 mM d-fructose. To increase the intracellular concentration of the substrate, the glucose/fructose facilitator gene from Zymomonas mobilis was incorporated into the engineered strain. The results showed that the allitol yield was enhanced significantly to 16.53 g/l with a conversion rate of 92 %. Through optimizing conversion conditions, allitol was produced effectively on a large scale by the whole-cell biotransformation system; the yield reached 48.62 g/l when 500 mM d-fructose was used as the substrate. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | D-TAGATOSE 3-EPIMERASE ; WHOLE-CELL BIOTRANSFORMATION ; GLUCOSE-DEHYDROGENASE GENES ; AGGLOMERANS STRAIN 221E ; D-PSICOSE 3-EPIMERASE ; D-MANNITOL FORMATION ; D-FRUCTOSE ; ETHYL (S)-4-CHLORO-3-HYDROXYBUTANOATE ; RIBITOL DEHYDROGENASE ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000352860600001 |
| 源URL | [http://124.16.173.210/handle/834782/1459]  |
| 专题 | 天津工业生物技术研究所_功能糖与天然活性物质研究组 孙媛霞 _期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin Airport Econ Area, Tianjin 300308, Peoples R China 2.Shanxi Med Univ, Key Lab Cellular Physiol, Minist Educ, Taiyuan 030001, Shanxi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Yueming,Li, Hongyi,Liu, Pingping,et al. Construction of allitol synthesis pathway by multi-enzyme coexpression in Escherichia coli and its application in allitol production[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2015,42(5):661-669. |
| APA | Zhu, Yueming,Li, Hongyi,Liu, Pingping,Yang, Jiangang,Zhang, Xueli,&Sun, Yuanxia.(2015).Construction of allitol synthesis pathway by multi-enzyme coexpression in Escherichia coli and its application in allitol production.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,42(5),661-669. |
| MLA | Zhu, Yueming,et al."Construction of allitol synthesis pathway by multi-enzyme coexpression in Escherichia coli and its application in allitol production".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 42.5(2015):661-669. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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