Biosynthesis of L-Sorbose and L-Psicose Based on C-C Bond Formation Catalyzed by Aldolases in an Engineered Corynebacterium glutamicum Strain
文献类型:期刊论文
作者 | Yang, Jiangang; Li, Jitao; Men, Yan; Zhu, Yueming; Zhang, Ying; Sun, Yuanxia; Ma, Yanhe |
刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY |
出版日期 | 2015-07-01 |
卷号 | 81期号:13页码:4284-4294 |
英文摘要 | The property of loose stereochemical control at aldol products from aldolases helped to synthesize multiple polyhydroxylated compounds with nonnatural stereoconfiguration. In this study, we discovered for the first time that some fructose 1,6-diphosphate aldolases (FruA) and tagatose 1,6-diphosphate (TagA) aldolases lost their strict stereoselectivity when using L-glyceraldehyde and synthesized not only L-sorbose but also a high proportion of L-psicose. Among the aldolases tested, TagA from Bacillus licheniformis (BGatY) showed the highest enzyme activity with L-glyceraldehyde. Subsequently, a "one-pot" reaction based on BGatY and fructose-1-phosphatase (YqaB) generated 378 mg/liter L-psicose and 199 mg/liter L-sorbose from dihydroxyacetonephosphate (DHAP) and L-glyceraldehyde. Because of the high cost and instability of DHAP, a microbial fermentation strategy was used further to produce L-sorbose/L-psicose from glucose and L-glyceraldehyde, in which DHAP was obtained from glucose through the glycolytic pathway, and some recombination pathways based on FruA or TagA and YqaB were constructed in Escherichia coli and Corynebacterium glutamicum strains. After evaluation of different host cells and combinations of FruA or TagA with YqaB and optimization of gene expression, recombinant C. glutamicum strain WT(pXFTY) was selected and produced 2.53 g/liter total ketoses, with a yield of 0.50 g/g L-glyceraldehyde. Moreover, deletion of gene cgl0331, encoding the Zn-dependent alcohol dehydrogenase in C. glutamicum, was confirmed for the first time to significantly decrease conversion of L-glyceraldehyde to glycerol and to increase yield of target products. Finally, fed-batch culture of strain SY14(pXFTY) produced 3.5 g/liter L-sorbose and 2.3 g/liter L-psicose, with a yield of 0.61 g/g L-glyceraldehyde. This microbial fermentation strategy also could be applied to efficiently synthesize other L-sugars. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
关键词[WOS] | ONE-POT SYNTHESIS ; RARE SUGARS ; L-FRUCTOSE ; ORGANIC-SYNTHESIS ; ENZYME ; DEHYDROGENASE ; NUCLEOSIDES ; PHOSPHATE ; TAGATOSE ; VECTORS |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000355844800007 |
源URL | [http://124.16.173.210/handle/834782/1490] |
专题 | 天津工业生物技术研究所_功能糖与天然活性物质研究组 孙媛霞 _期刊论文 |
作者单位 | Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin, Peoples R China |
推荐引用方式 GB/T 7714 | Yang, Jiangang,Li, Jitao,Men, Yan,et al. Biosynthesis of L-Sorbose and L-Psicose Based on C-C Bond Formation Catalyzed by Aldolases in an Engineered Corynebacterium glutamicum Strain[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2015,81(13):4284-4294. |
APA | Yang, Jiangang.,Li, Jitao.,Men, Yan.,Zhu, Yueming.,Zhang, Ying.,...&Ma, Yanhe.(2015).Biosynthesis of L-Sorbose and L-Psicose Based on C-C Bond Formation Catalyzed by Aldolases in an Engineered Corynebacterium glutamicum Strain.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,81(13),4284-4294. |
MLA | Yang, Jiangang,et al."Biosynthesis of L-Sorbose and L-Psicose Based on C-C Bond Formation Catalyzed by Aldolases in an Engineered Corynebacterium glutamicum Strain".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 81.13(2015):4284-4294. |
入库方式: OAI收割
来源:天津工业生物技术研究所
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