Functional Characterization of Cucurbitadienol Synthase and Triterpene Glycosyltransferase Involved in Biosynthesis of Mogrosides from Siraitia grosvenorii
文献类型：期刊论文
| 作者 | Dai, Longhai; Liu, Can; Zhu, Yueming; Zhang, Jiangsheng; Men, Yan; Zeng, Yan; Sun, Yuanxia |
| 刊名 | PLANT AND CELL PHYSIOLOGY
 |
| 出版日期 | 2015-06-01 |
| 卷号 | 56期号:6页码:1172-1182 |
| 关键词 | Cucurbitadienol Cucurbitadienol synthase Glycosyltransferase Mogrosides Siraitia grosvenorii |
| 英文摘要 | Mogrosides, the major bioactive components isolated from the fruits of Siraitia grosvenorii, are a family of cucurbitane-type tetracyclic triterpenoid saponins that are used worldwide as high-potency sweeteners and possess a variety of notable pharmacological activities. Mogrosides are synthesized from 2,3-oxidosqualene via a series of reactions catalyzed by cucurbitadienol synthase (CbQ), Cyt P450s (P450s) and UDP glycosyltransferases (UGTs) in vivo. However, the relevant genes have not been characterized to date. In this study, we report successful identification of SgCbQ and UGT74AC1, which were previously predicted via RNA-sequencing (RNA-seq) and digital gene expression (DGE) profile analysis of the fruits of S. grosvenorii. SgCbQ was functionally characterized by expression in the lanosterol synthase-deficient yeast strain GIL77 and was found to accumulate cucurbitadienol as the sole product. UGT74AC1 was heterologously expressed in Escherichia coli as a His-tag protein and it showed specificity for mogrol by transfer of a glucose moiety to the C-3 hydroxyl to form mogroside IE by in vitro enzymatic activity assays. This study reports the identification of CbQ and glycosyltransferase from S. grosvenorii for the first time. The results also suggest that RNA-seq, combined with DGE profile analysis, is a promising approach for discovery of candidate genes involved in biosynthesis of triterpene saponins. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Plant Sciences ; Cell Biology |
| 研究领域[WOS] | Plant Sciences ; Cell Biology |
| 关键词[WOS] | LEGUME MEDICAGO-TRUNCATULA ; BETA-AMYRIN SYNTHASE ; OXIDOSQUALENE CYCLASES ; UDP-GLYCOSYLTRANSFERASES ; TRANSCRIPTOME ANALYSIS ; SAPONIN BIOSYNTHESIS ; CRYSTAL-STRUCTURES ; GENES ; GLUCOSYLTRANSFERASE ; GLYCOSYLATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000356182700012 |
| 源URL | [http://124.16.173.210/handle/834782/1498]  |
| 专题 | 天津工业生物技术研究所_功能糖与天然活性物质研究组 孙媛霞 _期刊论文 |
| 作者单位 | Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai, Longhai,Liu, Can,Zhu, Yueming,et al. Functional Characterization of Cucurbitadienol Synthase and Triterpene Glycosyltransferase Involved in Biosynthesis of Mogrosides from Siraitia grosvenorii[J]. PLANT AND CELL PHYSIOLOGY,2015,56(6):1172-1182. |
| APA | Dai, Longhai.,Liu, Can.,Zhu, Yueming.,Zhang, Jiangsheng.,Men, Yan.,...&Sun, Yuanxia.(2015).Functional Characterization of Cucurbitadienol Synthase and Triterpene Glycosyltransferase Involved in Biosynthesis of Mogrosides from Siraitia grosvenorii.PLANT AND CELL PHYSIOLOGY,56(6),1172-1182. |
| MLA | Dai, Longhai,et al."Functional Characterization of Cucurbitadienol Synthase and Triterpene Glycosyltransferase Involved in Biosynthesis of Mogrosides from Siraitia grosvenorii".PLANT AND CELL PHYSIOLOGY 56.6(2015):1172-1182. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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