Effect of pH on cellulase production and morphology of Trichoderma reesei and the application in cellulosic material hydrolysis
文献类型：期刊论文
| 作者 | Li, Chen; Yang, Zhenhua; Zhang, Ronglin He Can; Zhang, Dongyuan; Chen, Shulin; Ma, Lijuan |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2013-12-01 |
| 卷号 | 168期号:4页码:470-477 |
| 关键词 | Cellulase beta-Glucosidase Protein pH Hydrolysis |
| 英文摘要 | A low-cost of cellulase achieved through improving fermentation technology remains a key requirement for commercialization of cellulosic biofuels and biochemicals. pH plays a very important role in the process of cellulase synthesis by Trichoderma reesei. In this work, effects of pH on the production and production rates of three cellulase components (endoglucanase, exoglucanase, beta-glucosidase) and mycelial morphology were studied. Production rates of the cellulase components were kept highest and the mycelial morphology was maintained at the optimal status by developing a phased pH control strategy in order to improve cellulase production. Cellulase production in terms of filter paper activity and beta-glucosidase production in batch fermentation increased 17.6% and 22%. Saccharification efficiency of the enzyme obtained by pH control was evaluated by hydrolyzing pretreated corn cob. Saccharification yield increased significantly (up to 26.2%) compared with that without pH control. These results add new knowledge on approach for improving cellulase production. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | BETA-GLUCOSIDASE ; CONTINUOUS-CULTURE ; RUT C-30 ; C30 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000327843200025 |
| 源URL | [http://124.16.173.210/handle/834782/1231]  |
| 专题 | 天津工业生物技术研究所_工业生物系统工程实验室 陈树林_期刊论文 |
| 作者单位 | Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Engn, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Chen,Yang, Zhenhua,Zhang, Ronglin He Can,et al. Effect of pH on cellulase production and morphology of Trichoderma reesei and the application in cellulosic material hydrolysis[J]. JOURNAL OF BIOTECHNOLOGY,2013,168(4):470-477. |
| APA | Li, Chen,Yang, Zhenhua,Zhang, Ronglin He Can,Zhang, Dongyuan,Chen, Shulin,&Ma, Lijuan.(2013).Effect of pH on cellulase production and morphology of Trichoderma reesei and the application in cellulosic material hydrolysis.JOURNAL OF BIOTECHNOLOGY,168(4),470-477. |
| MLA | Li, Chen,et al."Effect of pH on cellulase production and morphology of Trichoderma reesei and the application in cellulosic material hydrolysis".JOURNAL OF BIOTECHNOLOGY 168.4(2013):470-477. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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