Detection and quantitation of lipid in the microalga Tetraselmis subcordiformis (Wille) Butcher with BODIPY 505/515 staining
文献类型：期刊论文
| 作者 | Xu, Dong1; Gao, Zhengquan2; Li, Feng1; Fan, Xiao1; Zhang, Xiaowen1; Ye, Naihao1; Mou, Shanli1; Liang, Chengwei3; Li, Demao4 |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 127页码:386-390 |
| 关键词 | Tetraselmis subcordiformis Biodiesel BODIPY 505/515 Lipid determination Nitrogen limitation |
| 英文摘要 | BODIPY 505/515, a lipophilic bright green fluorescent dye was tested for lipid detection in the microalga Tetraselmis subcordiformis. A concentration of 0.28 mu g ml(-1) and staining for 6 min was optimal. Lipid bodies stained with BODIPY505/515 had a characteristic green fluorescence. Their volumes were determined using the sphere volume formula. Lipid accumulation under different nitrogen concentrations was analyzed. With an increase in NaNO3 concentration from 0 to 240 mg L-1, the maximum algal concentration increased from 8.23 +/- 0.62 (x 10(5) cells ml(-1)) to 1.61 +/- 0.13 (x 10(6) cells ml(-1)), while the maximum volume of intracellular neutral lipid decreased from 9.78 +/- 1.77 mu m(3) cell(-1) to 6.00 +/- 0.59 mu m(3) cell(-1). A comparison of the lipid contents measured by BODIPY 505/515 staining and the gravimetric method showed a positive correlation coefficient of R-2 = 0.93. BODIPY 505/515 staining is a promising method in lipid quantitation in T. subcordiformis. (c) 2012 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | NILE RED ; ACCUMULATION ; NITROGEN ; GROWTH ; DROPLETS ; ACID |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000312926400052 |
| 源URL | [http://124.16.173.210/handle/834782/1287]  |
| 专题 | 天津工业生物技术研究所_工业生物系统工程实验室 陈树林_期刊论文 |
| 作者单位 | 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 2.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Shandong, Peoples R China 3.Qingdao Univ Sci & Technol, Qingdao 266042, Peoples R China 4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Engn, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Dong,Gao, Zhengquan,Li, Feng,et al. Detection and quantitation of lipid in the microalga Tetraselmis subcordiformis (Wille) Butcher with BODIPY 505/515 staining[J]. BIORESOURCE TECHNOLOGY,2013,127:386-390. |
| APA | Xu, Dong.,Gao, Zhengquan.,Li, Feng.,Fan, Xiao.,Zhang, Xiaowen.,...&Li, Demao.(2013).Detection and quantitation of lipid in the microalga Tetraselmis subcordiformis (Wille) Butcher with BODIPY 505/515 staining.BIORESOURCE TECHNOLOGY,127,386-390. |
| MLA | Xu, Dong,et al."Detection and quantitation of lipid in the microalga Tetraselmis subcordiformis (Wille) Butcher with BODIPY 505/515 staining".BIORESOURCE TECHNOLOGY 127(2013):386-390. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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