Construction of a plasmid for heterologous protein expression with a constitutive promoter in Trichoderma reesei
文献类型：期刊论文
| 作者 | He, Ronglin; Zhang, Can; Guo, Wei; Wang, Lixian; Zhang, Dongyuan; Chen, Shunlin |
| 刊名 | PLASMID
 |
| 出版日期 | 2013-11-01 |
| 卷号 | 70期号:3页码:425-429 |
| 关键词 | T. reesei rp2 Promoter Heterologus expression beta-Glucosidase |
| 英文摘要 | Trichoderma reesei is widely used as a host for homologus and heterologus protein expression because of its well-known capability of protein secretion, especially cellulases secretion. In this study, a binary vector which could be used for protein expression was constructed with a constitutive promoter of rp2 gene. This vector contained the expression cassette Prp2-target gene-Trp2 and hygromycin B selection marker based on pCAMBIA1300 for T-DNA random insertion. The feasibility of the promoter was determined by eGFP (enhanced green fluorescence protein) expression in T. reesei. For heterologus protein expression, the expression of bgla from Aspergillus niger in the transformant was 3 folds higher than that of the wild type strain. The results demonstrate that this constitutive promoter could be applied for protein expression and thus this protein express system may contribute to the industrial protein production. (C) 2013 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Genetics & Heredity |
| 研究领域[WOS] | Genetics & Heredity |
| 关键词[WOS] | MAGNAPORTHE-GRISEA ; GENE ; TRANSFORMATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000328175600015 |
| 源URL | [http://124.16.173.210/handle/834782/1296]  |
| 专题 | 天津工业生物技术研究所_工业生物系统工程实验室 陈树林_期刊论文 |
| 作者单位 | Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Ronglin,Zhang, Can,Guo, Wei,et al. Construction of a plasmid for heterologous protein expression with a constitutive promoter in Trichoderma reesei[J]. PLASMID,2013,70(3):425-429. |
| APA | He, Ronglin,Zhang, Can,Guo, Wei,Wang, Lixian,Zhang, Dongyuan,&Chen, Shunlin.(2013).Construction of a plasmid for heterologous protein expression with a constitutive promoter in Trichoderma reesei.PLASMID,70(3),425-429. |
| MLA | He, Ronglin,et al."Construction of a plasmid for heterologous protein expression with a constitutive promoter in Trichoderma reesei".PLASMID 70.3(2013):425-429. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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