Purification and characterization of a new beta-glucosidase from Penicillium piceum and its application in enzymatic degradation of delignified corn stover
文献类型:期刊论文
作者 | Gao, Le; Gao, Feng; Zhang, Dongyuan; Zhang, Can; Wu, Gaihong; Chen, Shulin |
刊名 | BIORESOURCE TECHNOLOGY
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出版日期 | 2013-11-01 |
卷号 | 147页码:658-661 |
关键词 | beta-1,4-Glucosidase PpCel3B Inducer Synergism |
英文摘要 | A new beta-glucosidase (Cel3B) was first isolated from cellulytic fungi, designated as PpCel3B. Although PpCel3B was classified to GH family 3 based on the homology sequence, PpCel3B had different biological functions in cellulose degradation and signaling molecules production. PpCel3B was constitutive and could form multiple soluble lignocellulose inducers for cellulase and hemicellulase synthesis via high tranglycosylation activity and new enzymatic activity. Moreover, PpCel3B showed apparent synergism with cellulases by removing several inhibitors. Supplementing low doses of PpCel3B (52 mu g/g substrate) increased saccharification efficiency of cellulase produced by Trichoderma reesei and Penicillium piceum by 15% and 35%, respectively on delignified corn stover. PpCel3B had important application in boosting cellulase yield and efficiency. Published by Elsevier Ltd. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
关键词[WOS] | TRICHODERMA-REESEI ; CELLOBIOHYDROLASE I ; MOLECULAR-CLONING ; HYDROLYSIS ; CELLULOSE ; EXPRESSION ; GENES ; TRANSGLYCOSYLATION ; SOFTWOOD ; BIOMASS |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000326660900092 |
源URL | [http://124.16.173.210/handle/834782/1297] ![]() |
专题 | 天津工业生物技术研究所_工业生物系统工程实验室 陈树林_期刊论文 |
作者单位 | Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Engn, Tianjin, Peoples R China |
推荐引用方式 GB/T 7714 | Gao, Le,Gao, Feng,Zhang, Dongyuan,et al. Purification and characterization of a new beta-glucosidase from Penicillium piceum and its application in enzymatic degradation of delignified corn stover[J]. BIORESOURCE TECHNOLOGY,2013,147:658-661. |
APA | Gao, Le,Gao, Feng,Zhang, Dongyuan,Zhang, Can,Wu, Gaihong,&Chen, Shulin.(2013).Purification and characterization of a new beta-glucosidase from Penicillium piceum and its application in enzymatic degradation of delignified corn stover.BIORESOURCE TECHNOLOGY,147,658-661. |
MLA | Gao, Le,et al."Purification and characterization of a new beta-glucosidase from Penicillium piceum and its application in enzymatic degradation of delignified corn stover".BIORESOURCE TECHNOLOGY 147(2013):658-661. |
入库方式: OAI收割
来源:天津工业生物技术研究所
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