Kinetic studies on batch cultivation of Trichoderma reesei and application to enhance cellulase production by fed-batch fermentation
文献类型：期刊论文
| 作者 | Ma, Lijuan; Li, Chen; Yang, Zhenhua; Jia, Wendi; Zhang, Dongyuan; Chen, Shulin |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2013-07-20 |
| 卷号 | 166期号:4页码:192-197 |
| 关键词 | Cellulase Trichoderma reesei Kinetic models Mass balance Fed-batch fermentation |
| 英文摘要 | Reducing the production cost of cellulase as the key enzyme for cellulose hydrolysis to fermentable sugars remains a major challenge for biofuel production. Because of the complexity of cellulase production, kinetic modeling and mass balance calculation can be used as effective tools for process design and optimization. In this study, kinetic models for cell growth, substrate consumption and cellulase production in batch fermentation were developed, and then applied in fed-batch fermentation to enhance cellulase production. Inhibition effect of substrate was considered and a modified Luedeking-Piret model was developed for cellulase production and substrate consumption according to the growth characteristics of Trichoderma reesei. The model predictions fit well with the experimental data. Simulation results showed that higher initial substrate concentration led to decrease of cellulase production rate. Mass balance and kinetic simulation results were applied to determine the feeding strategy. Cellulase production and its corresponding productivity increased by 82.13% after employing the proper feeding strategy in fed-batch fermentation. This method combining mathematics and chemometrics by kinetic modeling and mass balance can not only improve cellulase fermentation process, but also help to better understand the cellulase fermentation process. The model development can also provide insight to other similar fermentation processes. (c) 2013 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | RUT C30 ; CONTINUOUS-CULTURE ; ENZYME ; OPTIMIZATION ; IMPROVEMENT ; MORPHOLOGY ; SUBSTRATE ; PROTEIN ; GROWTH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000321749500007 |
| 源URL | [http://124.16.173.210/handle/834782/1298]  |
| 专题 | 天津工业生物技术研究所_工业生物系统工程实验室 陈树林_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Engn, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Lijuan,Li, Chen,Yang, Zhenhua,et al. Kinetic studies on batch cultivation of Trichoderma reesei and application to enhance cellulase production by fed-batch fermentation[J]. JOURNAL OF BIOTECHNOLOGY,2013,166(4):192-197. |
| APA | Ma, Lijuan,Li, Chen,Yang, Zhenhua,Jia, Wendi,Zhang, Dongyuan,&Chen, Shulin.(2013).Kinetic studies on batch cultivation of Trichoderma reesei and application to enhance cellulase production by fed-batch fermentation.JOURNAL OF BIOTECHNOLOGY,166(4),192-197. |
| MLA | Ma, Lijuan,et al."Kinetic studies on batch cultivation of Trichoderma reesei and application to enhance cellulase production by fed-batch fermentation".JOURNAL OF BIOTECHNOLOGY 166.4(2013):192-197. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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