Effect of earlier unfolded protein response and efficient protein disposal system on cellulase production in Rut C30
文献类型：期刊论文
| 作者 | Wang, Guokun1,2; Zhang, Dongyuan1; Chen, Shulin1 |
| 刊名 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2014-10-01 |
| 卷号 | 30期号:10页码:2587-2595 |
| 关键词 | Cellulase QM9414 Rut C30 Trichoderma reesei Unfolded protein response |
| 英文摘要 | Trichoderma reesei (T. reesei) has been widely used in production of cellulolytic enzymes and heterologous proteins because of its high secretion capacity. The lack of knowledge on protein secretion mechanisms, however, still hinders rational improvement on cellulase production. The transcript levels of cellulases and components involved in post-transcriptional procedures were compared in this study between two mutants, QM9414 and Rut C30 for evaluating the effects of modification and secretion upon cellulase production. The results showed that cellulase induction by cellulose drastically up-regulated expressions of the sensor of unfolded protein, chaperone and folding-assisted enzymes in endoplasmic reticulum and resulted in unfolded protein response (UPR) and low-grade increase in secretory transporters' expression similar to that of chemical treatment. Rut C30 demonstrated earlier and more sustainable expressions of elements involved in UPR and lower amount of cellular retained cellulase compared to QM9414, indicating that Rut C30 had hypercellulolytic property partially for its earlier and enhanced UPR to more efficiently dispose of protein. Modifying post-translational peptides and enhancing protein flux to avoid protein accumulation during cellulase production may be a feasible approach for strain improvement. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | FUNGUS TRICHODERMA-REESEI ; HYPOCREA-JECORINA ; ENDOPLASMIC-RETICULUM ; FILAMENTOUS FUNGI ; SECRETED PROTEINS ; STRAIN RUT-C30 ; GENE ; PATHWAY ; VIRIDE ; IMPROVEMENT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000341635000005 |
| 源URL | [http://124.16.173.210/handle/834782/1330]  |
| 专题 | 天津工业生物技术研究所_工业生物系统工程实验室 陈树林_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biosyst & Bioproc Engn, Tianjin 300308, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Guokun,Zhang, Dongyuan,Chen, Shulin. Effect of earlier unfolded protein response and efficient protein disposal system on cellulase production in Rut C30[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2014,30(10):2587-2595. |
| APA | Wang, Guokun,Zhang, Dongyuan,&Chen, Shulin.(2014).Effect of earlier unfolded protein response and efficient protein disposal system on cellulase production in Rut C30.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,30(10),2587-2595. |
| MLA | Wang, Guokun,et al."Effect of earlier unfolded protein response and efficient protein disposal system on cellulase production in Rut C30".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 30.10(2014):2587-2595. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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