Construction of a constitutively expressed homo-fermentative pathway in Lactobacillus brevis
文献类型：期刊论文
| 作者 | Guo, Wei; He, Ronglin; Ma, Lijuan; Jia, Wendi; Li, Demao; Chen, Shulin |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2014-08-01 |
| 卷号 | 98期号:15页码:6641-6650 |
| 关键词 | Lactobacillus brevis Homo-fermentative Lactic acid Fructose-6-phosphate kinase Fructose-1,6-biphosphate aldolase |
| 英文摘要 | Lactobacillus brevis is a promising lactic acid producing strain that simultaneously utilizes glucose and xylose from lignocellulosic hydrolysate without carbon catabolic repression and inhibition. The production of by-products acetic acid and ethanol has been the major drawback of this strain. Two genes, pfkA (fructose-6-phosphate kinase [PFK]) and fbaA (fructose-1,6-biphosphate aldolase [FBA]), that encode the key enzymes of the EMP/glycolytic pathway from Lactobacillus rhamnosus, were fused to the downstream of the strong promoter P32 and expressed in L. brevis s3f4 as a strategy to minimize the formation of by-products. By expressing the two enzymes, a homo-fermentative pathway for lactic acid production was constructed. The lactic acid yields achieved from glucose in the transformants were 1.12 and 1.16 mol/mol, which is higher than that of the native strain (0.74 mol/mol). However, the lactic acid yield from xylose in the transformants stayed the same as that of the native strain. Enzyme assay indicated that the activity of the foreign protein FBA in the transformants was much higher than that of the native strains, but was ten times lower than that in L. rhamnosus. This result was consistent with the metabolic flux analysis, which indicated that the conversion efficiency of the expressed PFK and FBA was somewhat low. Less than 20 % of the carbons accumulated in the form of fructose-6-phosphate were converted into glyceraldehyde-3-phosphate (GAP) by the expressed PFK and FBA. Metabolic flux analysis also indicated that the enzyme phosphoketolase (XPK) played an important role in splitting the carbon flow from the pentose phosphate pathway to the phosphoketolase pathway. This study suggested that the lactic acid yield of L. brevis could be improved by constructing a homo-fermentative pathway. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | L-LACTIC ACID ; XYLOSE ; SUGARS ; FRUCTOSE ; BIOMASS ; PENTOSE ; HEXOSE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000339880300010 |
| 源URL | [http://124.16.173.210/handle/834782/1392]  |
| 专题 | 天津工业生物技术研究所_工业生物系统工程实验室 陈树林_期刊论文 |
| 作者单位 | Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind BioSyst & Bioproc Engn, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Wei,He, Ronglin,Ma, Lijuan,et al. Construction of a constitutively expressed homo-fermentative pathway in Lactobacillus brevis[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2014,98(15):6641-6650. |
| APA | Guo, Wei,He, Ronglin,Ma, Lijuan,Jia, Wendi,Li, Demao,&Chen, Shulin.(2014).Construction of a constitutively expressed homo-fermentative pathway in Lactobacillus brevis.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,98(15),6641-6650. |
| MLA | Guo, Wei,et al."Construction of a constitutively expressed homo-fermentative pathway in Lactobacillus brevis".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 98.15(2014):6641-6650. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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