Biochemical characterization of a new beta-glucosidase (Cel3E) from Penicillium piceum and its application in boosting lignocelluloses bioconversion and forming disaccharide inducers: New insights into the role of beta-glucosidase
文献类型：期刊论文
| 作者 | Gao, Le1; Gao, Feng1; Jiang, Xukai2; Zhang, Can1; Zhang, Dongyuan1; Wang, Lushan2; Wu, Gaihong1; Chen, Shulin1 |
| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2014-05-01 |
| 卷号 | 49期号:5页码:768-774 |
| 关键词 | beta-1,4-glucosidase PpCel3E Inducer Synergism Transglycosylation |
| 英文摘要 | Fungal genome sequencing has revealed the presence of multiple putative beta-glucosidases; however, information regarding these new beta-glucosidases is limited. A new beta-glucosidase from Penicillium piceum, designated as PpCel3E, was first isolated and characterized. Using p-nitrophenyl-beta-D-glucoside as substrate, PpCel3E showed the lowest Km among the P-glucosidases among all fungi studied. Moreover, PpCel3E exhibited a high transglycosylation activity of 1100mg gentiobiose/mg and 142 mg sophorose/mg using glucose as the donor. PpCel3E is a novel bifunctional glycoside hydrolase with both P-glucosidase and P-xylosidase activity. Our results show that PpCel3E plays an important role in forming soluble cellulose inducer compounds, as well as in amplifying weak cellulase inducer signal and hemicellulase synthesis via its high transglycosylation activity. Supplementing PpCel3E at low concentrations (40 mu g/g substrate) increased the saccharification efficiency of different cellulases by 20% to 27% by removing multiple inhibitors. 2014 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | TRICHODERMA-REESEI ; TALAROMYCES-EMERSONII ; FILAMENTOUS FUNGUS ; MOLECULAR-CLONING ; DEGRADING ENZYMES ; HYPOCREA-JECORINA ; CELLULASE ; CELLOBIOHYDROLASE ; GENE ; PURIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000336111700007 |
| 源URL | [http://124.16.173.210/handle/834782/1416]  |
| 专题 | 天津工业生物技术研究所_工业生物系统工程实验室 陈树林_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Eng, Tianjin, Peoples R China 2.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Le,Gao, Feng,Jiang, Xukai,et al. Biochemical characterization of a new beta-glucosidase (Cel3E) from Penicillium piceum and its application in boosting lignocelluloses bioconversion and forming disaccharide inducers: New insights into the role of beta-glucosidase[J]. PROCESS BIOCHEMISTRY,2014,49(5):768-774. |
| APA | Gao, Le.,Gao, Feng.,Jiang, Xukai.,Zhang, Can.,Zhang, Dongyuan.,...&Chen, Shulin.(2014).Biochemical characterization of a new beta-glucosidase (Cel3E) from Penicillium piceum and its application in boosting lignocelluloses bioconversion and forming disaccharide inducers: New insights into the role of beta-glucosidase.PROCESS BIOCHEMISTRY,49(5),768-774. |
| MLA | Gao, Le,et al."Biochemical characterization of a new beta-glucosidase (Cel3E) from Penicillium piceum and its application in boosting lignocelluloses bioconversion and forming disaccharide inducers: New insights into the role of beta-glucosidase".PROCESS BIOCHEMISTRY 49.5(2014):768-774. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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