Characterization of the Interactions between Polyethylene Glycol and Cellulase during the Hydrolysis of Lignocellulose
文献类型：期刊论文
| 作者 | Zong, Zhiyou1; Ma, Lijuan1; Yu, Liang2; Zhang, Dongyuan1; Yang, Zhenhua1; Chen, Shulin1 |
| 刊名 | BIOENERGY RESEARCH
 |
| 出版日期 | 2015-03-01 |
| 卷号 | 8期号:1页码:270-278 |
| 关键词 | Lignocellulose Cellulase Polyethylene glycol Hydrolysis Entropy generation Hydrogen bonds |
| 英文摘要 | The interaction between polyethylene glycol (PEG) and the substrate has been widely examined for the purpose of using it to improve the enzymatic hydrolysis efficiency of lignocellulosic biomass. However, the knowledge about the positive effect of PEG on cellulase remains very limited and the mechanism underlying this positive effect has not been clearly elucidated. Our study found that the conversion rates of corn stover and Avicel PH101 increased to 53.8 and 86.8 %, respectively, with the pretreatment using PEG 6000 prior to hydrolysis. After incubation for 48 h, 68.2 % of the enzymatic activity was retained in the solution, whereas in the control, only 38.6 % of the enzyme activity was retained. The existence of this interaction and entropy generation was qualitatively confirmed by calorimetric titration. Moreover, entropy was identified as a reason for the hydrolytic acceleration. Additionally, hydrophobic effects and the hydrogen bonds formed between PEG and key amino acids contributed to cellulase activity enhancement. These results provide insight into the observed positive effects of PEG on cellulase function and indicate that PEG pretreatment would be beneficial to reduce enzyme load. |
| WOS标题词 | Science & Technology ; Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Energy & Fuels ; Environmental Sciences |
| 研究领域[WOS] | Energy & Fuels ; Environmental Sciences & Ecology |
| 关键词[WOS] | ENHANCING ENZYMATIC-HYDROLYSIS ; TRICHODERMA-REESEI ; ETHANOL-PRODUCTION ; CORN STOVER ; POLY(ETHYLENE GLYCOL) ; NONIONIC SURFACTANT ; CRYSTAL-STRUCTURE ; BETA-GLUCOSIDASE ; PRETREATMENT ; CELLOBIOHYDROLASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000350051300023 |
| 源URL | [http://124.16.173.210/handle/834782/1430]  |
| 专题 | 天津工业生物技术研究所_工业生物系统工程实验室 陈树林_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Engn, Tianjin, Peoples R China 2.Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA |
| 推荐引用方式 GB/T 7714 | Zong, Zhiyou,Ma, Lijuan,Yu, Liang,et al. Characterization of the Interactions between Polyethylene Glycol and Cellulase during the Hydrolysis of Lignocellulose[J]. BIOENERGY RESEARCH,2015,8(1):270-278. |
| APA | Zong, Zhiyou,Ma, Lijuan,Yu, Liang,Zhang, Dongyuan,Yang, Zhenhua,&Chen, Shulin.(2015).Characterization of the Interactions between Polyethylene Glycol and Cellulase during the Hydrolysis of Lignocellulose.BIOENERGY RESEARCH,8(1),270-278. |
| MLA | Zong, Zhiyou,et al."Characterization of the Interactions between Polyethylene Glycol and Cellulase during the Hydrolysis of Lignocellulose".BIOENERGY RESEARCH 8.1(2015):270-278. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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