Enhancement of dihydroxyacetone production by a mutant of Gluconobacter oxydans
文献类型：期刊论文
| 作者 | Ma, Lijuan1,2; Lu, Wenyu1,3; Xia, Zhendong1; Wen, Jianping1,3 |
| 刊名 | BIOCHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2010-03-15 |
| 卷号 | 49期号:1页码:61-67 |
| 关键词 | He-Ne laser irradiation Bioconversion 1,3-Dihydroxyacetone Glycerol Enzyme activity Kinetics |
| 英文摘要 | To improve the yield of 1,3-dihydroxyacetone(DHA) and the bioconversion rate of glycerol by Gluconobacter oxydans(G. oxydans), He-Ne laser irradiation technology was employed in this work. Under the optimal irradiation doses of 21 mW, 21 min, mutant G. oxydans GM51 with high DHA production capacity and stable heredity was obtained through tolerance tests to glycerol and DHA. Activity of the key enzyme, glycerol dehydrogenase, in GM51 was 5.01 U/mL after 30h culture, 75.17% higher than that in the wild (2.86 U/mL) with the same initial glycerol concentration of 100 g/L The culture time of GM51 had been shortened by 16 h (conventional culture time is 58 h). DHA yield of CM51 was up to 91.5%, increased by 77.6% with an improvement of DHA productivity from 1.29 to 2.29 g L(-1) h(-1) when cultivated in 7 L bioreactor. Moreover, comparative studies on kinetics of the mutant and wild further confirmed the decrease in inhibitory of substrate and product through He-Ne laser irradiation. The mutagenesis mechanism was speculated as the mutation of the gene encoding glycerol dehydrogenase, which led to improvement in the activity of this enzyme. Therefore, He-Ne laser irradiation could efficiently enhance the DHA production ability of G. oxydans. (C) 2009 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | NE-LASER IRRADIATION ; MICROBIAL-PRODUCTION ; ESCHERICHIA-COLI ; GLYCEROL ; CONVERSION ; CELLS ; OPTIMIZATION ; EXPRESSION ; GROWTH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275581400009 |
| 源URL | [http://124.16.173.210/handle/834782/1468]  |
| 专题 | 天津工业生物技术研究所_工业生物系统工程实验室 陈树林_期刊论文 |
| 作者单位 | 1.Tianjin Univ, Sch Chem Engn & Technol, Dept Biochem Engn, Tianjin 300072, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Tianjin Univ, Key Lab Syst Bioengn, Minist Educ, Tianjin 300072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Lijuan,Lu, Wenyu,Xia, Zhendong,et al. Enhancement of dihydroxyacetone production by a mutant of Gluconobacter oxydans[J]. BIOCHEMICAL ENGINEERING JOURNAL,2010,49(1):61-67. |
| APA | Ma, Lijuan,Lu, Wenyu,Xia, Zhendong,&Wen, Jianping.(2010).Enhancement of dihydroxyacetone production by a mutant of Gluconobacter oxydans.BIOCHEMICAL ENGINEERING JOURNAL,49(1),61-67. |
| MLA | Ma, Lijuan,et al."Enhancement of dihydroxyacetone production by a mutant of Gluconobacter oxydans".BIOCHEMICAL ENGINEERING JOURNAL 49.1(2010):61-67. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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