Linker length and flexibility induces new cellobiohydrolase activity of PoCel6A from Penicillium oxalicum
文献类型：期刊论文
| 作者 | Gao, Le1,2; Wang, Lushan1; Jiang, Xukai1; Qu, Yinbo1 |
| 刊名 | BIOTECHNOLOGY JOURNAL
 |
| 出版日期 | 2015-06-01 |
| 卷号 | 10期号:6页码:899-904 |
| 关键词 | Cel6A linker flexiblity linker length Penicillium oxalicum |
| 英文摘要 | In a previous study, a novel cellobiohydrolase, PoCel6A, with new enzymatic activity against p-nitrophenyl--D-cellobioside (pNPC), was purified from Penicillium oxalicum. The cellulose-binding module and catalytic domain of PoCel6A showed a high degree of sequence similarity with other fungal Cel6As. However, PoCel6A had 11 more amino acids in the linker region than other Cel6As. To evaluate the relationship between the longer linker of PoCel6A and its enzymatic activity, 11 amino acids were deleted from the linker region of PoCel6A. The shortened PoCel6A linker nullified the enzymatic activity against pNPC but dramatically increased the enzyme's capacity for crystalline cellulose degradation. The shortened linker segment appeared to have no effect on the secondary structural conformation of PoCel6A. Another variant (PoCel6A-6pro) with six consecutive proline residues in the interdomain linker had a higher rigid linker, and no enzymatic activity was observed against soluble and insoluble substrate. The flexibility of the linker had an important function in the formation of active cellulase. The length and flexibility of the linker is clearly able to modify the function of PoCel6A and induce new characteristics of Cel6A. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | CELLULASE ; DECUMBENS ; PURIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000355845400007 |
| 源URL | [http://124.16.173.210/handle/834782/1488]  |
| 专题 | 天津工业生物技术研究所_工业生物系统工程实验室 陈树林_期刊论文 |
| 作者单位 | 1.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Engn, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Le,Wang, Lushan,Jiang, Xukai,et al. Linker length and flexibility induces new cellobiohydrolase activity of PoCel6A from Penicillium oxalicum[J]. BIOTECHNOLOGY JOURNAL,2015,10(6):899-904. |
| APA | Gao, Le,Wang, Lushan,Jiang, Xukai,&Qu, Yinbo.(2015).Linker length and flexibility induces new cellobiohydrolase activity of PoCel6A from Penicillium oxalicum.BIOTECHNOLOGY JOURNAL,10(6),899-904. |
| MLA | Gao, Le,et al."Linker length and flexibility induces new cellobiohydrolase activity of PoCel6A from Penicillium oxalicum".BIOTECHNOLOGY JOURNAL 10.6(2015):899-904. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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