Protein disulfide isomerase homolog TrPDI2 contributing to cellobiohydrolase production in Trichoderma reesei
文献类型：期刊论文
| 作者 | Wang, Guokun1,2; Lv, Pin1; He, Ronglin1; Wang, Haijun1; Wang, Lixian1; Zhang, Dongyuan1; Chen, Shulin1 |
| 刊名 | ENZYME AND MICROBIAL TECHNOLOGY
 |
| 出版日期 | 2015-09-01 |
| 卷号 | 77页码:21-28 |
| 关键词 | Cellobiohydrolase Cellulase Disulfide bond Protein disulfide isomerase Trichoderma reesei |
| 英文摘要 | The majority of the cysteine residues in the secreted proteins form disulfide bonds via protein disulfide isomerase (PDI)-mediated catalysis, stabilizing the enzyme activity. The role of PDI in cellulase production is speculative, as well as the possibility of PDI as a target for improving enzyme production efficiency of Trichoderma reesei, a widely used producer of enzyme for the production of lignocellulose-based biofuels and biochemicals. Here, we report that a PDI homolog,TrPDI2 in T. reesei exhibited a 36.94% and an 11.81% similarity to Aspergillus niger TIGA and T. reesei PDI1,respectively. The capability of TrPDI2 to recover the activity of reduced and denatured RNase by promoting refolding verified its protein disulfide isomerase activity. The overexpression of Trpdi2 increased the secretion and the activity of CBH1 at the early stage of cellulase induction. In addition, both the expression level and redox state of TrPDI2 responded to cellulase induction in T. reesei, providing sustainable oxidative power to ensure cellobiohydrolase maturation and production. The results suggest that TrPDI2 may contribute to cellobiohydrolase secretion by enhancing the capability of disulfide bond formation, which is essential for protein folding and maturation. (C) 2015 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | ENDOPLASMIC-RETICULUM ; TRANSFORMATION SYSTEM ; GENE ; ENDOGLUCANASE ; EFFICIENT ; RUT-C30 ; STRESS ; STRAIN ; C30 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000358267900004 |
| 源URL | [http://124.16.173.210/handle/834782/1503]  |
| 专题 | 天津工业生物技术研究所_工业生物系统工程实验室 陈树林_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, PR, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Guokun,Lv, Pin,He, Ronglin,et al. Protein disulfide isomerase homolog TrPDI2 contributing to cellobiohydrolase production in Trichoderma reesei[J]. ENZYME AND MICROBIAL TECHNOLOGY,2015,77:21-28. |
| APA | Wang, Guokun.,Lv, Pin.,He, Ronglin.,Wang, Haijun.,Wang, Lixian.,...&Chen, Shulin.(2015).Protein disulfide isomerase homolog TrPDI2 contributing to cellobiohydrolase production in Trichoderma reesei.ENZYME AND MICROBIAL TECHNOLOGY,77,21-28. |
| MLA | Wang, Guokun,et al."Protein disulfide isomerase homolog TrPDI2 contributing to cellobiohydrolase production in Trichoderma reesei".ENZYME AND MICROBIAL TECHNOLOGY 77(2015):21-28. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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