Ethanol induced astaxanthin accumulation and transcriptional expression of carotenogenic genes in Haematococcus pluvialis
文献类型：期刊论文
| 作者 | Wen, Zewen1; Liu, Zhiyong1; Hou, Yuyong1; Liu, Chenfeng1; Gao, Feng1; Zheng, Yubin2; Chen, Fangjian1 |
| 刊名 | ENZYME AND MICROBIAL TECHNOLOGY
 |
| 出版日期 | 2015-10-01 |
| 卷号 | 78页码:10-17 |
| 关键词 | Haematococcus pluvialis Astaxanthin Ethanol ipi Fed-batch induction |
| 英文摘要 | Haematococcus pluvialis is one of the most promising natural sources of astaxanthin. However, inducing the accumulation process has become one of the primary obstacles in astaxanthin production. In this study, the effect of ethanol on astaxanthin accumulation was investigated. The results demonstrated that astaxanthin accumulation occurred with ethanol addition even under low-light conditions. The astaxanthin productivity could reach 11.26 mg L-1 d(-1) at 3% (v/v) ethanol, which was 2.03 times of that of the control. The transcriptional expression patterns of eight carotenogenic genes were evaluated using real-time PCR. The results showed that ethanol greatly enhanced transcription of the isopentenyl diphosphate (IPP) isomerase genes (ipi-1 and ipi-2), which were responsible for isomerization reaction of IPP and dimethylallyl diphosphate (DMAPP). This finding suggests that ethanol induced astaxanthin biosynthesis was up-regulated mainly by ipi-1 and ipi-2 at transcriptional level, promoting isoprenoid synthesis and substrate supply to carotenoid formation. Thus ethanol has the potential to be used as an effective reagent to induce astaxanthin accumulation in H. pluvialis. (C) 2015 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | DIFFERENTIAL EXPRESSION ; OXIDATIVE STRESS ; BIOSYNTHESIS ; INDUCTION ; OXYGEN ; CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000359500300002 |
| 源URL | [http://124.16.173.210/handle/834782/1514]  |
| 专题 | 天津工业生物技术研究所_工业生物系统工程实验室 陈树林_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Engn, Tianjin 300308, Peoples R China 2.Shandong Jinjing Biotechnol Co Ltd, Jinjing 261313, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wen, Zewen,Liu, Zhiyong,Hou, Yuyong,et al. Ethanol induced astaxanthin accumulation and transcriptional expression of carotenogenic genes in Haematococcus pluvialis[J]. ENZYME AND MICROBIAL TECHNOLOGY,2015,78:10-17. |
| APA | Wen, Zewen.,Liu, Zhiyong.,Hou, Yuyong.,Liu, Chenfeng.,Gao, Feng.,...&Chen, Fangjian.(2015).Ethanol induced astaxanthin accumulation and transcriptional expression of carotenogenic genes in Haematococcus pluvialis.ENZYME AND MICROBIAL TECHNOLOGY,78,10-17. |
| MLA | Wen, Zewen,et al."Ethanol induced astaxanthin accumulation and transcriptional expression of carotenogenic genes in Haematococcus pluvialis".ENZYME AND MICROBIAL TECHNOLOGY 78(2015):10-17. |
入库方式： OAI收割
来源：天津工业生物技术研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。