Computer-Assisted Rational Modifications to Improve the Thermostability of beta-Glucosidase from Penicillium piceum H16
文献类型：期刊论文
| 作者 | Zong, Zhiyou1; Gao, Le1; Cai, Wensheng1,2; Yu, Liang3; Cui, Chao; Chen, Shulin1; Zhang, Dongyuan1 |
| 刊名 | BIOENERGY RESEARCH
 |
| 出版日期 | 2015-09-01 |
| 卷号 | 8期号:3页码:1384-1390 |
| 关键词 | beta-Glucosidase Thermostability Directed evolution Virtual mutation Molecular dynamics |
| 英文摘要 | beta-Glucosidase plays key role in the saccharification of cellulosic biomass. The use of beta-glucosidase would benefit from improvements in its thermostability in some industrial applications. In this work, two rational computer-aided strategies were devised to enhance the thermostability of a highly active beta-glucosidase from Penicillium piceum H16. Single-point mutants were proposed based on the proline theory. The effects of the mutation on protein stability were further predicted using molecular dynamics (MD) simulations. The optimal single mutant, G305P, was found to provide a 20.0 % improvement in thermostability. Then computer-assisted virtual saturated mutation was first used to select the optimal triple mutations which would further increase the thermostability of beta-glucosidase by the Calculate Mutation Energy/Stability module of Discovery Studio (DS) 4.0. The triple mutant, S507F/Q512W/S514W, provided a 46.3 % improvement in thermostability. A detailed structural analysis showed that the additional hydrophobic interactions played decisive roles in the improvement in thermostability, and the use of mutants with enhanced stability may benefit formulation of more effective enzyme cocktails for lignocellulosic bioconversion. |
| WOS标题词 | Science & Technology ; Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Energy & Fuels ; Environmental Sciences |
| 研究领域[WOS] | Energy & Fuels ; Environmental Sciences & Ecology |
| 关键词[WOS] | SITE-DIRECTED MUTAGENESIS ; ENHANCED THERMOSTABILITY ; STABILITY ; PROLINE ; MUTANTS ; GLYCINE ; DESIGN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000360770400044 |
| 源URL | [http://124.16.173.210/handle/834782/1524]  |
| 专题 | 天津工业生物技术研究所_工业生物系统工程实验室 陈树林_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Engn, Tianjin, Peoples R China 2.Nankai Univ, Coll Chem, Tianjin 300071, Peoples R China 3.Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA |
| 推荐引用方式 GB/T 7714 | Zong, Zhiyou,Gao, Le,Cai, Wensheng,et al. Computer-Assisted Rational Modifications to Improve the Thermostability of beta-Glucosidase from Penicillium piceum H16[J]. BIOENERGY RESEARCH,2015,8(3):1384-1390. |
| APA | Zong, Zhiyou.,Gao, Le.,Cai, Wensheng.,Yu, Liang.,Cui, Chao.,...&Zhang, Dongyuan.(2015).Computer-Assisted Rational Modifications to Improve the Thermostability of beta-Glucosidase from Penicillium piceum H16.BIOENERGY RESEARCH,8(3),1384-1390. |
| MLA | Zong, Zhiyou,et al."Computer-Assisted Rational Modifications to Improve the Thermostability of beta-Glucosidase from Penicillium piceum H16".BIOENERGY RESEARCH 8.3(2015):1384-1390. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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