An ethanolamine kinase Eki1 affects radial growth and cell wall integrity in Trichoderma reesei
文献类型：期刊论文
| 作者 | He, Ronglin1,2,3; Guo, Wei1; Zhang, Dongyuan1 |
| 刊名 | FEMS MICROBIOLOGY LETTERS
 |
| 出版日期 | 2015-09-01 |
| 卷号 | 362期号:17 |
| 关键词 | ethanolamine kinase conidiation cell wall integrity maintenance ethanolamine accumulation |
| 英文摘要 | Ethanolamine kinase (ATP: ethanolamine O-phosphotransferase, EC 2.7.1.82) catalyzes the committed step of phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway. The functions of eki genes that encode ethanolamine kinase have been intensively studied in mammalian cells, fruit flies and yeast. However, the role of the eki gene has not yet been characterized in filamentous fungi. In this study, Treki1, an ortholog of Saccharomyces cerevisiae EKI1, was identified and functionally characterized using a target gene deletion strategy in Trichoderma reesei. A Treki deletion mutant was less sensitive to cell wall stressors calcofluor white and Congo red and released fewer protoplasts during cell wall digestion than the parent strain QM9414. Further transcription analysis showed that the expression levels of five genes that encode chitin synthases were drastically increased in the Delta Treki1 mutant. The chitin content was also increased in the null mutant of Treki1 comparing to the parent strain. In addition, the Delta Treki1 mutant exhibited defects in radial growth, conidiation and the accumulation of ethanolamine. The results indicate that Treki1 plays a key role in growth and development and in the maintenance of cell wall integrity in T. reesei. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | YEAST SACCHAROMYCES-CEREVISIAE ; PHOSPHOLIPID-SYNTHESIS ; MAGNAPORTHE-ORYZAE ; GENE-EXPRESSION ; IDENTIFICATION ; REGULATOR ; PATHWAY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000361788500005 |
| 源URL | [http://124.16.173.210/handle/834782/1577]  |
| 专题 | 天津工业生物技术研究所_工业生物系统工程实验室 陈树林_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Tianjin Univ Sci & Technol, Minist Educ, Key Lab Ind Fermentat Microbiol, Tianjin 300457, Peoples R China 3.Tianjin Univ Sci & Technol, Tianjin Key Lab Ind Microbiol, Tianjin 300457, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Ronglin,Guo, Wei,Zhang, Dongyuan. An ethanolamine kinase Eki1 affects radial growth and cell wall integrity in Trichoderma reesei[J]. FEMS MICROBIOLOGY LETTERS,2015,362(17). |
| APA | He, Ronglin,Guo, Wei,&Zhang, Dongyuan.(2015).An ethanolamine kinase Eki1 affects radial growth and cell wall integrity in Trichoderma reesei.FEMS MICROBIOLOGY LETTERS,362(17). |
| MLA | He, Ronglin,et al."An ethanolamine kinase Eki1 affects radial growth and cell wall integrity in Trichoderma reesei".FEMS MICROBIOLOGY LETTERS 362.17(2015). |
入库方式： OAI收割
来源：天津工业生物技术研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。