Unique Genomic Arrangements in an Invasive Serotype M23 Strain of Streptococcus pyogenes Identify Genes That Induce Hypervirulence
文献类型：期刊论文
| 作者 | Bao, Yunjuan1,2,3; Liang, Zhong1,2; Booyjzsen, Claire1,2; Mayfield, Jeffrey A.1,2; Li, Yang3; Lee, Shaun W.4; Ploplis, Victoria A.1,2; Song, Hui3; Castellino, Francis J.1,2 |
| 刊名 | JOURNAL OF BACTERIOLOGY
 |
| 出版日期 | 2014-12-01 |
| 卷号 | 196期号:23页码:4089-4102 |
| 英文摘要 | The first genome sequence of a group A Streptococcus pyogenes serotype M23 (emm23) strain (M23ND), isolated from an invasive human infection, has been completed. The genome of this opacity factor-negative (SOF-) strain is composed of a circular chromosome of 1,846,477 bp. Gene profiling showed that this strain contained six phage-encoded and 24 chromosomally inherited well-known virulence factors, as well as 11 pseudogenes. The bacterium has acquired four large prophage elements, Phi M23ND. 1 to Phi M23ND. 4, harboring genes encoding streptococcal superantigen (ssa), streptococcal pyrogenic exotoxins (speC, speH, and speI), and DNases (spd1 and spd3), with phage integrase genes being present at one flank of each phage insertion, suggesting that the phages were integrated by horizontal gene transfer. Comparative analyses revealed unique large-scale genomic rearrangements that result in genomic rearrangements that differ from those of previously sequenced GAS strains. These rearrangements resulted in an imbalanced genomic architecture and translocations of chromosomal virulence genes. The covS sensor in M23ND was identified as a pseudogene, resulting in the attenuation of speB function and increased expression of the genes for the chromosomal virulence factors multiple-gene activator (mga), Mprotein (emm23), C5a peptidase (scpA), fibronectinbinding proteins (sfbI and fbp54), streptolysin O (slo), hyaluronic acid capsule (hasA), streptokinase (ska), and DNases (spd and spd3), which were verified by PCR. These genes are responsible for facilitating host epithelial cell binding and and/or immune evasion, thus further contributing to the virulence of M23ND. In conclusion, strain M23ND has become highly pathogenic as the result of a combination of multiple genetic factors, particularly gene composition and mutations, prophage integrations, unique genomic rearrangements, and regulated expression of critical virulence factors. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | GROUP-A STREPTOCOCCI ; FIBRONECTIN-BINDING PROTEIN ; EXTRACELLULAR CYSTEINE PROTEASE ; CLUSTER 2B STREPTOKINASE ; M1T1 CLONE ; EXPERIMENTAL ANTICANCER ; VIRULENCE REGULON ; EPITHELIAL-CELLS ; RHEUMATIC-FEVER ; VIR REGULON |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000344645500011 |
| 源URL | [http://124.16.173.210/handle/834782/1368]  |
| 专题 | 天津工业生物技术研究所_基因工程与微生物应用技术研究组 宋恢_期刊论文 |
| 作者单位 | 1.Univ Notre Dame, WM Keck Ctr Transgene Res, Notre Dame, IN 46556 USA 2.Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin, Peoples R China 4.Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA |
| 推荐引用方式 GB/T 7714 | Bao, Yunjuan,Liang, Zhong,Booyjzsen, Claire,et al. Unique Genomic Arrangements in an Invasive Serotype M23 Strain of Streptococcus pyogenes Identify Genes That Induce Hypervirulence[J]. JOURNAL OF BACTERIOLOGY,2014,196(23):4089-4102. |
| APA | Bao, Yunjuan.,Liang, Zhong.,Booyjzsen, Claire.,Mayfield, Jeffrey A..,Li, Yang.,...&Castellino, Francis J..(2014).Unique Genomic Arrangements in an Invasive Serotype M23 Strain of Streptococcus pyogenes Identify Genes That Induce Hypervirulence.JOURNAL OF BACTERIOLOGY,196(23),4089-4102. |
| MLA | Bao, Yunjuan,et al."Unique Genomic Arrangements in an Invasive Serotype M23 Strain of Streptococcus pyogenes Identify Genes That Induce Hypervirulence".JOURNAL OF BACTERIOLOGY 196.23(2014):4089-4102. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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