Improvement of Antimicrobial Activity of Pediocin PA-1 by Site-directed Mutagenesis in C-terminal Domain
文献类型：期刊论文
| 作者 | Sun, Lin1,2; Song, Hui1; Zheng, Wen1 |
| 刊名 | PROTEIN AND PEPTIDE LETTERS
 |
| 出版日期 | 2015 |
| 卷号 | 22期号:11页码:1007-1012 |
| 关键词 | Antimicrobial activity C-terminal domain hairpin-like structure pediocin PA-1 site-directed mutagenesis |
| 英文摘要 | Pediocin PA-1 is a well-known Class IIa bacteriocin which shows strong inhibitory effect against Listeria monocytogenes. In this work, in order to improve the antimicrobial activity, eight single-site mutants and six combination mutants on nine interesting sites in the C-terminal region of pediocin PA-1 were constructed and expressed in Escherichia coli heterologously. Ten mutants demonstrated enhancement activity when performed the agar diffusion test to the indicator strain L. monocytogenes. The substitution of glycine in position 29 to alanine showed the most distinct increase of antimicrobial activity which clarified that 29G acted as a significant role as to guide the pediocin PA-1 molecule to dip into receptor membrane. Combination mutants of sites 29G to 32A illustrated that a hydrophobic tip of the hairpin-like structure and a smaller bundle of the alpha-helix domain facilitate the penetrating of pediocin PA-1 into a hydrophobic domain of the membrane-embedded subunits of the mannose-phosphotransferase system (MPTs). |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | CLASS IIA BACTERIOCINS ; LISTERIA-MONOCYTOGENES ; MUTATIONAL ANALYSIS ; ESCHERICHIA-COLI ; PEPTIDES ; RESIDUES ; RECOGNITION ; SPECIFICITY ; COMPONENT ; SPECTRUM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000362117100007 |
| 源URL | [http://124.16.173.210/handle/834782/1565]  |
| 专题 | 天津工业生物技术研究所_基因工程与微生物应用技术研究组 宋恢_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Lin,Song, Hui,Zheng, Wen. Improvement of Antimicrobial Activity of Pediocin PA-1 by Site-directed Mutagenesis in C-terminal Domain[J]. PROTEIN AND PEPTIDE LETTERS,2015,22(11):1007-1012. |
| APA | Sun, Lin,Song, Hui,&Zheng, Wen.(2015).Improvement of Antimicrobial Activity of Pediocin PA-1 by Site-directed Mutagenesis in C-terminal Domain.PROTEIN AND PEPTIDE LETTERS,22(11),1007-1012. |
| MLA | Sun, Lin,et al."Improvement of Antimicrobial Activity of Pediocin PA-1 by Site-directed Mutagenesis in C-terminal Domain".PROTEIN AND PEPTIDE LETTERS 22.11(2015):1007-1012. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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