One-Pot Biosynthesis of High-Concentration alpha-Glucose 1-Phosphate from Starch by Sequential Addition of Three Hyperthermophilic Enzymes
文献类型：期刊论文
| 作者 | Zhou, Wei1; You, Chun2; Ma, Hongwu1; Ma, Yanhe1; Zhang, Y. -H. Percival1,2,3 |
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| 出版日期 | 2016-03-02 |
| 卷号 | 64期号:8页码:1777-1783 |
| 关键词 | Camellia sinensis hydroperoxide lyase Ectropis obliqua suppressive subtractive hybridization (SSH) induced defense response |
| 英文摘要 | Hydroperoxide lyase (HPL, E.C. 4.1.2.) is the major enzyme in the biosynthesis of natural volatile aldehydes and alcohols in plants, however, little was known about HPL in tea plants (Camellia sinensis). A unique cDNA fragment was isolated by suppressive subtractive hybridization (SSH) from a tea plant subjected to herbivory by tea geometrid Ectropis obliqua. This full length cDNA acquired by RACE was 1476 bp and encoded 491 amino acids. DNA and protein BLAST searches showed high homology to HPL sequences from other plants. The His-tag expression vector pET-32a(+)/CsHPL was constructed and transferred into Escherichia coli Rosetta (DE3). The expression product of recombinant CsHPL in E. coli was about 60 kDa. The enzyme activity of CsHPL was 0.20 mu mol.min(-1).mg(-1). Quantitative RT-PCR analysis indicated CsHPL was strongly up-regulated in tea plants after Ectropis obliqua attack, suggesting that it may be an important candidate for defense against insects in tea plants. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
| 研究领域[WOS] | Agriculture ; Chemistry ; Food Science & Technology |
| 关键词[WOS] | ARCHAEON THERMOCOCCUS-LITORALIS ; ESCHERICHIA-COLI ; MALTODEXTRIN-PHOSPHORYLASE ; GLUCAN PHOSPHORYLASE ; THERMOSTABLE 4-ALPHA-GLUCANOTRANSFERASE ; LEUCONOSTOC-MESENTEROIDES ; SUCROSE PHOSPHORYLASE ; THERMUS-THERMOPHILUS ; PYROCOCCUS-FURIOSUS ; SULFOLOBUS-TOKODAII |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000371454000015 |
| 源URL | [http://124.16.173.210/handle/834782/1527]  |
| 专题 | 天津工业生物技术研究所_计算生物设计 马洪武 _期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, 32 West Seventh Ave, Tianjin 300308, Peoples R China 2.Cell Free Bioinnovat Inc, 1800 Kraft Dr,Suite 222, Blacksburg, VA 24060 USA 3.Virginia Tech, Dept Biol Syst Engn, 304 Seitz Hall, Blacksburg, VA 24061 USA |
| 推荐引用方式 GB/T 7714 | Zhou, Wei,You, Chun,Ma, Hongwu,et al. One-Pot Biosynthesis of High-Concentration alpha-Glucose 1-Phosphate from Starch by Sequential Addition of Three Hyperthermophilic Enzymes[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2016,64(8):1777-1783. |
| APA | Zhou, Wei,You, Chun,Ma, Hongwu,Ma, Yanhe,&Zhang, Y. -H. Percival.(2016).One-Pot Biosynthesis of High-Concentration alpha-Glucose 1-Phosphate from Starch by Sequential Addition of Three Hyperthermophilic Enzymes.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,64(8),1777-1783. |
| MLA | Zhou, Wei,et al."One-Pot Biosynthesis of High-Concentration alpha-Glucose 1-Phosphate from Starch by Sequential Addition of Three Hyperthermophilic Enzymes".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 64.8(2016):1777-1783. |
入库方式： OAI收割
来源：天津工业生物技术研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。