Structural basis of the substrate specificity of the FPOD/FAOD family revealed by fructosyl peptide oxidase from Eupenicillium terrenum
文献类型：期刊论文
| 作者 | Gan, Weiqiong1,2; Gao, Feng1; Xing, Keke3; Jia, Minze1; Liu, Haiping3; Gong, Weimin1,4 |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
 |
| 出版日期 | 2015-04-01 |
| 卷号 | 71页码:381-387 |
| 关键词 | fructosyl peptide oxidase Eupenicillium terrenum FAOD/FPOD family |
| 英文摘要 | The FAOD/FPOD family of proteins has the potential to be useful for the longterm detection of blood glucose levels in diabetes patients. A bottleneck for this application is to find or engineer a FAOD/FPOD family enzyme that is specifically active towards alpha-fructosyl peptides but is inactive towards other types of glycated peptides. Here, the crystal structure of fructosyl peptide oxidase from Eupenicillium terrenum (EtFPOX) is reported at 1.9 angstrom resolution. In contrast to the previously reported structure of amadoriase II, EtFPOX has an open substrate entrance to accommodate the large peptide substrate. The functions of residues critical for substrate selection are discussed based on structure comparison and sequence alignment. This study reveals the first structural details of group I FPODs that prefer alpha-fructosyl substrates and could provide significant useful information for uncovering the mechanism of substrate specificity of FAOD/FPODs and guidance towards future enzyme engineering for diagnostic purposes. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 关键词[WOS] | AMINO-ACID OXIDASE ; SITE-DIRECTED MUTAGENESIS ; ASPERGILLUS-SP ; FUSARIUM-OXYSPORUM ; MOLECULAR-CLONING ; AMADORIASE-II ; EC 1.5.3 ; EXPRESSION ; PURIFICATION ; OXIDOREDUCTASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000352508000003 |
| 源URL | [http://124.16.173.210/handle/834782/1451]  |
| 专题 | 天津工业生物技术研究所_结构生物化学研究 龚为民 _期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Key Lab RNA, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ctr Chem Biol, Tianjin 300457, Peoples R China 4.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gan, Weiqiong,Gao, Feng,Xing, Keke,et al. Structural basis of the substrate specificity of the FPOD/FAOD family revealed by fructosyl peptide oxidase from Eupenicillium terrenum[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2015,71:381-387. |
| APA | Gan, Weiqiong,Gao, Feng,Xing, Keke,Jia, Minze,Liu, Haiping,&Gong, Weimin.(2015).Structural basis of the substrate specificity of the FPOD/FAOD family revealed by fructosyl peptide oxidase from Eupenicillium terrenum.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,71,381-387. |
| MLA | Gan, Weiqiong,et al."Structural basis of the substrate specificity of the FPOD/FAOD family revealed by fructosyl peptide oxidase from Eupenicillium terrenum".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 71(2015):381-387. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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