Increased production of alkaline polygalacturonate lyase in the recombinant Pichia pastoris by controlling cell concentration during continuous culture
文献类型：期刊论文
| 作者 | Wang, Huilin1,2; Li, Jianghua3; Liu, Long3; Li, Xiaoman1,2; Jia, Dongxu3; Du, Guocheng3,4; Chen, Jian3,4; Song, Jiangning1,2,5 |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2012-11-01 |
| 卷号 | 124页码:338-346 |
| 关键词 | Pichia pastoris Alkaline polygalacturonate lyase (PGL) Cell concentration Continuous culture Productivity |
| 英文摘要 | Recombinant alkaline polygalacturonate lyase (PGL) production by recombinant Pichia pastoris GS115 was selected as a model to study as a continuous culture strategy for enhancing heterologous protein production based on controlling methanol feeding (CCCM culture) or on dual carbon source feeding (CCCD culture). Using the CCCM process with a dry cell weight of 75 g/L regulated by controlling methanol concentration in the induction media, the final PGL activity was 441.9 U/mL. The PGL productivity (Q(v)) and the average specific enzyme production rate (Q(x)) were 4.65 U mL(-1) h(-1) and 84.5 U g(-1) 11(-1), an increase of 42.1% and 191.2%, respectively, over what was achieved with traditional fed-batch culture with high cell density. The control strategies also reduced proteolytic degradation by 84.1% in the fermentation broth and increased cell viability by 12.2%. (C) 2012 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | TRANSIENT CONTINUOUS CULTURES ; HUMAN SERUM-ALBUMIN ; PROTEIN-PRODUCTION ; ALCOHOL OXIDASE ; MUT(+) STRAIN ; MIXED FEEDS ; METHANOL ; FERMENTATION ; EXPRESSION ; YEAST |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000311175000045 |
| 源URL | [http://124.16.173.210/handle/834782/1323]  |
| 专题 | 天津工业生物技术研究所_结构生物信息学和整合系统生物学实验室 宋江宁_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214122, Peoples R China 4.Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China 5.Monash Univ, Dept Biochem & Mol Biol, Fac Med, Melbourne, Vic 3800, Australia |
| 推荐引用方式 GB/T 7714 | Wang, Huilin,Li, Jianghua,Liu, Long,et al. Increased production of alkaline polygalacturonate lyase in the recombinant Pichia pastoris by controlling cell concentration during continuous culture[J]. BIORESOURCE TECHNOLOGY,2012,124:338-346. |
| APA | Wang, Huilin.,Li, Jianghua.,Liu, Long.,Li, Xiaoman.,Jia, Dongxu.,...&Song, Jiangning.(2012).Increased production of alkaline polygalacturonate lyase in the recombinant Pichia pastoris by controlling cell concentration during continuous culture.BIORESOURCE TECHNOLOGY,124,338-346. |
| MLA | Wang, Huilin,et al."Increased production of alkaline polygalacturonate lyase in the recombinant Pichia pastoris by controlling cell concentration during continuous culture".BIORESOURCE TECHNOLOGY 124(2012):338-346. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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